PMID: 6978079Apr 1, 1982Paper

Ionic requirements for H+ secretion and membrane elaboration in frog oxyntic cells

The American Journal of Physiology
C D Logsdon, T E Machen

Abstract

We have tested whether the ions K+, Na+, or Cl- are required for either initiation or maintenance of secretory parameters of amphibian oxyntic cells. In vitro mucosal preparations were washed repeatedly in Ringer solution lacking the ion in question either before (i.e., resting tissues) or after addition of stimulants. Electrophysiology, H+ secretion, and morphology (morphometric analysis of oxyntic cells) were monitored. Na+ or K+. Stimulation of resting tissues in Na+ or K+-free solutions elicited no H+ secretion; there was only a small increase (approximately twofold) in secretory surface density (Sv) (during control Sv increased sixfold). The Na+-free effects were partially reversed by high [K+]. In secreting tissues, Na+ or K+-free solutions caused H+ secretion to decrease to zero. Gastric glands from these mucosae appeared "occluded": the gland lumen was filled with apposed apical projections of oxyntic cells. Cl-. Stimulation after Cl- removal initiated low levels of H+ secretion. Morphologically the cells either appeared normally stimulated (Sv increased eightfold) or, more rarely, took on the occluded appearance. Cl- removal from stimulated mucosae decreased H+ secretion approximately 50%, but the cells were normally e...Continue Reading

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