PMID: 3942249Jan 1, 1986

Ionic requirements for intracellular pH regulation in rainbow trout hepatocytes

The American Journal of Physiology
P J Walsh

Abstract

The ionic requirements for pH regulation in isolated rainbow trout (Salmo gairdneri) hepatocytes were determined by manipulation of intracellular pH (pHi; measured by the dimethyloxazolidinedione distribution technique) by NH4Cl prepulse and changes in external [CO2] in the presence and absence of various drugs and external ions. The presence of a Na+/H+(NH+4) exchange system is supported by the following results: 1) the rate of recovery from an acid load is decreased by amiloride (0.5 mM) or reduction of external [Na+]; 2) the rate of 22Na uptake is increased during recovery from an acid load, and this increase in amiloride sensitive. The presence of a Cl-/HCO3- exchange system is supported by the observations that 1) pHi is increased, and 2) rates of recovery of pHi from acid loading are enhanced, by exposure to 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (0.5 mM) and reductions in external [Cl-]. Further studies are required to determine the role of these exchange processes during physiological pHi perturbations.

Citations

May 21, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hannes L EbnerGerhard Krumschnabel

Related Concepts

Acids
Carbonic Acid Ions
Body Fluids
Chloride Ion Level
Hydrogen
Hydrogen-Ion Concentration
Intracellular Fluid
Ion Exchange
Ions
Liver

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