Ionic transport in the plasma membrane of carrot protoplasts from embryogenic cell-suspension cultures

FEBS Letters
M BreganteF LoSchiavo

Abstract

Ionic transport properties of protoplasts obtained from embryogenic carrot suspension cells were studied by the patch-clamp technique. In the whole-cell configuration, carrot protoplasts presented macroscopic time-dependent outward currents, showing kinetics of activation which did not depend appreciably on the amplitude of the stimulus. Time- and voltage-dependent whole-cell inward rectifying currents as well as instantaneous non-selective currents were also observed. Both time-dependent inward and outward currents are carried by potassium ions. In a cell-attached configuration, two types of single-channel signals, displaying conductances of 10 and 17 pS, were observed; the instantaneous 10 pS channel was also present in outside-out excised patches.

References

Feb 1, 1991·The Journal of Membrane Biology·K A Ketchum, R J Poole
Apr 1, 1991·The Journal of Membrane Biology·K FairleyN A Walker
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J I Schroeder, S Hagiwara
Nov 1, 1988·The Journal of General Physiology·J I Schroeder
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1994·European Biophysics Journal : EBJ·T RinkG Boheim
Jan 1, 1994·The Plant Journal : for Cell and Molecular Biology·M R Blatt, G Thiel
Jan 1, 1995·Plant Physiology·W. H. Wu, S. M. Assmann
Jul 1, 1994·Plant Physiology·L. GirominiR. Colombo
Nov 1, 1988·Plant Physiology·N MoranR L Satter
Dec 1, 1988·Planta·D S BushR L Jones
Jan 1, 1984·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G GiulianoM Terzi

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Citations

Aug 29, 2006·FEBS Letters·Elide FormentinFiorella Lo Schiavo

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