Ionically crosslinked Ad/chitosan nanocomplexes processed by electrospinning for targeted cancer gene therapy

Journal of Controlled Release : Official Journal of the Controlled Release Society
Yeonah ParkChae-Ok Yun

Abstract

For effective cancer gene therapy, systemic administration of tumor-targeting adenoviral (Ad) complexes is critical for delivery to both primary and metastatic lesions. Electrospinning was used to generate nanocomplexes of Ad, chitosan, poly(ethylene glycol) (PEG), and folic acid (FA) for effective FA receptor-expressing tumor-specific transduction. The chemical structure of the Ad/chitosan-PEG-FA nanocomplexes was characterized by NMR and FT-IR, and the diameter and surface charge were analyzed by dynamic light scattering and zeta potentiometry, respectively. The average size of Ad/chitosan-PEG-FA nanocomplexes was approximately 140 nm, and the surface charge was 2.1 mV compared to -4.9 mV for naked Ad. Electron microscopy showed well-dispersed, individual Ad nanocomplexes without aggregation or degradation. Ad/chitosan nanocomplexes retained biological activity without impairment of the transduction efficiency of naked Ad. The transduction efficiency of Ad/chitosan-PEG-FA was increased as a function of FA ratio in FA receptor-expressing KB cells, but not in FA receptor-negative U343 cells, demonstrating FA receptor-targeted viral transduction. In addition, the transduction efficiency of Ad/chitosan-PEG-FA was 57.2% higher tha...Continue Reading

References

May 23, 2001·Critical Reviews in Oncology/hematology·E GalanisS J Russell
Oct 25, 2002·Human Gene Therapy·Linda J Obenauer-KutnerMichael J Grace
Jul 5, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Julia LanciottiCatherine O'Riordan
Dec 9, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hoyin MokMichael A Barry
May 26, 2005·Journal of Colloid and Interface Science·Jeongsoo SuhMansoo Choi
Jun 7, 2005·Biotechnology and Bioengineering·Gary K LeeDavid V Schaffer
Dec 27, 2005·Carbohydrate Research·Lei Li, You-Lo Hsieh
May 18, 2006·Bioconjugate Chemistry·In Kyung OhTae Gwan Park
Oct 19, 2006·Journal of the National Cancer Institute·Joo-Hang KimChae-Ok Yun
Jul 14, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jian-Qing GaoShinsaku Nakagawa
Sep 15, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yukyung JungSeungjoo Haam
Mar 4, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sascha MaretschekThomas Kissel
Aug 4, 2009·Advanced Drug Delivery Reviews·Jun-Hyeog JangHae-Won Kim
Dec 17, 2009·Advanced Drug Delivery Reviews·Kerry D Fisher, Leonard W Seymour

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Citations

May 30, 2014·International Journal of Nanomedicine·Ghulam JalaniDong Woo Lim
Nov 2, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Tony Shing Chau LiKoichi Honke
Jul 28, 2012·Therapeutic Delivery·Gareth R WilliamsChristopher J Branford-White
Apr 9, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oh-Joon KwonChae-Ok Yun
May 9, 2012·Advanced Drug Delivery Reviews·Amy J HallidayMark J Cook
Jan 4, 2012·Advanced Drug Delivery Reviews·Joung-Woo ChoiChae-Ok Yun
Jun 28, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oh-Joon KwonChae-Ok Yun
Oct 11, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joung-Woo ChoiSung Wan Kim
Oct 2, 2012·Acta Biomaterialia·Mahrokh DadsetanMichael J Yaszemski
Feb 14, 2012·International Journal of Pharmaceutics·Hongkwan ParkJung Hyun Kim
Dec 31, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jaesung KimSung Wan Kim
Jan 21, 2016·Expert Opinion on Drug Delivery·Lan MeiGang Guo
Nov 5, 2016·Colloids and Surfaces. B, Biointerfaces·Xingzhen ZhangGuangxi Zhai
Aug 15, 2018·Journal of Drug Targeting·Sweet NaskarSuraj Sharma
Oct 1, 2019·Annales pharmaceutiques françaises·Sopan NangareTejashwini Muthane
Mar 28, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Sima Emadi AllahyariFarhad Jadidi-Niaragh
May 10, 2021·International Journal of Biological Macromolecules·Parvaneh Ghaderi Shikhi-Abadi, Mohammad Irani

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