Ion/ion proton-transfer kinetics: implications for analysis of ions derived from electrospray of protein mixtures

Analytical Chemistry
Scott A McLuckeyK G Asano

Abstract

Protein ions of different mass and charge but similar mass-to-charge ratios are shown to undergo significantly different rates of differential neutralization, defined as the rate of change of charge with time, upon initiation of reactions with oppositely charged ions in the quadrupole ion trap. Overlapping charge state distributions arising from mixtures of ions of dissimilar charge are separated on the mass-to-charge scale at short reactions times. It is also demonstrated that the time frame for near total neutralization, defined as charge reduction to the 1+ ion, is relatively insensitive to initial charge state. It is shown, for example, that the (M + 11H)(11+)-(M + 22H)22+ ions derived from horse skeletal muscle apomyoglobin yield the (M + H)+ ion as the major ion/ion reaction product over the same reaction period that largely converts doubly protonated bradykinin to the singly protonated species. Less than 25% of the bradykinin ions are expected to be totally neutralized when roughly 7% of the myoglobin ions are expected to be totally neutralized. The phenomenon of significantly different initial differential neutralization rates for ions of dissimilar charge, and the relative insensitivity to ion charge for total neutrali...Continue Reading

References

Jul 1, 1990·Analytical Chemistry·C F Ijames, C L Wilkins
Nov 15, 1996·Analytical Chemistry·J L Stephenson, S A McLuckey
Oct 6, 1989·Science·B F Chmelka, A Pines
Mar 1, 1992·Journal of the American Society for Mass Spectrometry·B B Reinhold, V N Reinhold

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Citations

Jun 9, 1999·Mass Spectrometry Reviews·S A McLuckey, J L Stephenson
Apr 24, 2013·Journal of the American Society for Mass Spectrometry·Christopher M RoseJohn E P Syka
Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Yu XiaJames W Hager
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Brian L FreyLloyd M Smith
Oct 10, 2008·Journal of the American Society for Mass Spectrometry·Joshua F Emory, Scott A McLuckey
Oct 12, 2010·Journal of the American Society for Mass Spectrometry·J Larry Campbell, J C Yves Le Blanc
Feb 19, 2002·Current Opinion in Biotechnology·James L StephensonJonathan L Bundy
Oct 30, 1998·Journal of the American Society for Mass Spectrometry·S J ValentineD E Clemmer
Sep 8, 2005·Journal of the American Chemical Society·Harsha P GunawardenaScott A McLuckey
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·John E P SykaDonald F Hunt
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joshua J CoonDonald F Hunt
Nov 22, 2015·Journal of the American Society for Mass Spectrometry·Nicholas M RileyJoshua J Coon
Jan 7, 2009·Rapid Communications in Mass Spectrometry : RCM·Joshua F EmoryScott A McLuckey
Jul 7, 2007·Journal of Mass Spectrometry : JMS·Jeremiah J BowersScott A McLuckey
Jun 27, 2015·Journal of the American Society for Mass Spectrometry·Christopher M RoseJoshua J Coon
Sep 2, 2015·Journal of the American Society for Mass Spectrometry·Kenneth J Laszlo, Matthew F Bush
Jun 9, 2016·Journal of Mass Spectrometry : JMS·Stella K BetancourtScott A McLuckey
Jul 12, 2016·Journal of the American Chemical Society·Kenneth J LaszloMatthew F Bush
Aug 4, 2007·Molecular & Cellular Proteomics : MCP·David M GoodJoshua J Coon
Jun 16, 2006·BioTechniques·David M Good, Joshua J Coon
Jul 19, 2002·Journal of Mass Spectrometry : JMS·Gavin E Reid, Scott A McLuckey
Dec 22, 2012·Chemical Communications : Chem Comm·Boone M Prentice, Scott A McLuckey
Sep 25, 2009·Analytical Chemistry·Vincent LarrailletJérôme Lemoine
Apr 16, 2021·Analytical Chemistry·Julia R Bonney, Boone M Prentice
Jan 29, 2002·Analytical Chemistry·Scott A McLuckeyJ Mitchell Wells
Feb 14, 2004·Analytical Chemistry·Peng PanScott A McLuckey
Aug 9, 2019·Journal of the American Society for Mass Spectrometry·Scott A UgrinDonald F Hunt
Feb 23, 2017·Journal of the American Society for Mass Spectrometry·Kenneth J LaszloMatthew F Bush
Nov 7, 2019·Analytical Chemistry·David Foreman, Scott A McLuckey
Jan 9, 2004·Analytical Chemistry·Peng Pan, Scott A McLuckey
Jun 12, 2003·Journal of the American Chemical Society·J Mitchell WellsScott A McLuckey
Sep 18, 2004·Analytical Chemistry·Jin WuScott A McLuckey
Feb 3, 2000·Analytical Chemistry·M ScalfL M Smith
Dec 26, 2001·Chemical Reviews·C S Hoaglund-HyzerD E Clemmer

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