Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro

Journal of Radiation Research
Agata AbramowiczMonika Pietrowska

Abstract

Exosomes and other extracellular vesicles are key players in cell-to-cell communication, and it has been proposed that they are involved in different aspects of the response to ionizing radiation, including transmitting the radiation-induced bystander effect and mediating radioresistance. The functional role of exosomes depends on their molecular cargo, including proteome content. Here we aimed to establish the proteome profile of exosomes released in vitro by irradiated UM-SCC6 cells derived from human head-and-neck cancer and to identify processes associated with radiation-affected proteins. Exosomes and other small extracellular vesicles were purified by size-exclusion chromatography from cell culture media collected 24 h after irradiation of cells with a single 2, 4 or 8 Gy dose, and then proteins were identified using a shotgun LC-MS/MS approach. Exosome-specific proteins encoded by 1217 unique genes were identified. There were 472 proteins whose abundance in exosomes was significantly affected by radiation (at any dose), including 425 upregulated and 47 downregulated species. The largest group of proteins affected by radiation (369 species) included those with increased abundance at all radiation doses (≥2 Gy). Several ge...Continue Reading

References

May 3, 2006·Cancer Research·Xin YuArnold J Levine
Sep 10, 2008·Proteomics·Richard J SimpsonJustin W E Lim
Oct 3, 2008·Cancer Research·Brian D LehmannDavid M Terrian
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Apr 21, 2009·Nature Reviews. Cancer·Kevin M Prise, Joe M O'Sullivan
May 1, 2010·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Mark D HurwitzAlexzander Asea
May 23, 2012·Radiation Research·Ammar H J Al-MayahMunira A Kadhim
Aug 21, 2012·The International Journal of Biochemistry & Cell Biology·Claudia Bang, Thomas Thum
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 21, 2013·BMC Bioinformatics·Michal MarczykJoanna Polanska
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·David G MeckesNancy Raab-Traub
Jan 28, 2014·Translational Oncology·W Tris ArscottKevin A Camphausen
Apr 29, 2014·Seminars in Cancer Biology·Carolina Villarroya-BeltriMaría Mittelbrunn
Mar 17, 2015·Mutation Research·Ammar Al-MayahMunira Kadhim
Dec 29, 2015·Oncology Reports·Wiktoria M Suchorska, Michal S Lach
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
Apr 28, 2016·Protein and Peptide Letters·Karol JelonekMonika Pietrowska
May 17, 2017·Nature Communications·Akiko TakahashiEiji Hara
Feb 8, 2018·Frontiers in Immunology·Katalin LumniczkyBenjamin Frey

❮ Previous
Next ❯

Citations

Jan 10, 2020·Experimental & Molecular Medicine·Ramesh KakarlaYong-Joon Chwae
May 10, 2020·Radiation Research·Agata AbramowiczPiotr Widłak
Sep 27, 2019·Frontiers in Oncology·Jie NiYong Li
Apr 2, 2020·International Journal of Molecular Sciences·Simone MoertlMichael J Atkinson
Sep 25, 2019·International Journal of Molecular Sciences·Nils LudwigTorsten E Reichert
Nov 9, 2019·International Journal of Molecular Sciences·Tünde SzatmáriKatalin Lumniczky
Jun 11, 2020·International Journal of Molecular Sciences·Linda HofmannMarie-Nicole Theodoraki
Nov 8, 2020·International Journal of Molecular Sciences·Xinyu QuKatie Meehan
Apr 27, 2021·Journal of Radiation Research·Rodrigo BerzaghiInigo Martinez-Zubiaurre
May 28, 2021·Breast Cancer Research and Treatment·Maryam FeghhiNasrollah Jabbari
Aug 8, 2021·International Journal of Molecular Sciences·Mihaela TudorFrançois Chevalier

❮ Previous
Next ❯

Methods Mentioned

BETA
size exclusion chromatography
Protein Assay
electrophoresis
transmission electron microscopy
dynamic light scattering
protein folding

Software Mentioned

Mascot engine
Gene
Malvern Zetasizer
Protein Discoverer
Proteome Discoverer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.