Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: repair of double-strand breaks in deoxyribonucleic acid.

Journal of Bacteriology
K M UlmerA J Sinskey

Abstract

The extremely gentle lysis and unfolding procedures that have been developed for the isolation of nucleoid deoxyribonucleic acid (DNA; K. M. Ulmer et al., J. Bacteriol. 138:475-485, 1979) yield undamaged, replicating genomes, thus permitting direct measurement of the formation and repair of DNA double-strand breaks at biologically significant doses of ionizing radiation. Repair of ionizing radiation damage to folded chromosomes of Escherichia coli K-12 strain AB2497 was observed within 2 to 3 h of post-irradiation incubation in growth medium. Such behavior was not observed after post-irradiation incubation in growth medium of a recA13 strain (strain AB2487). A model based on recombinational repair is proposed to explain the formation of 2,200 to 2,300S material during early stages of incubation and to explain subsequent changes in the gradient profiles. Association of unrepaired DNA with the plasma membrane is proposed to explain the formation of a peak of rapidly sedimenting material (greater than 3,100S) during the later stages of repair. Direct evidence of repair of double-strand breaks during post-irradiation incubation in growth medium was obtained from gradient profiles of DNA from ribonuclease-digested chromosomes. The s...Continue Reading

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Citations

Jul 25, 2007·FEMS Microbiology Letters·David J Schlesinger
Jul 1, 1991·Physics in Medicine and Biology·V Michalik
Mar 1, 1981·Critical Reviews in Toxicology·P F Schendel
May 1, 1995·Molecular Microbiology·A Kuzminov
Nov 25, 2018·Genes·Belén Mendoza-ChamizoGodefroid Charbon
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Kuzminov
Aug 1, 1980·Applied and Environmental Microbiology·J R PellonR F Gomez
Dec 9, 1993·Clinical Materials·I P MatthewsA H Samuel

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