Ionizing radiation-induced gene modulations, cytokine content changes and telomere shortening in mouse fetuses exhibiting forelimb defects

Developmental Biology
Hanane DerradjiSarah Baatout

Abstract

Several lines of evidence have linked limb teratogenesis to radiation-induced apoptosis and to the p53 status in murine fetuses. In previous reports, we studied the occurrence of various malformations after intrauterine irradiation and showed that these malformations were modulated by p53-deficiency as well as by the developmental stage at which embryos were irradiated. In this new study, we focused onto one particular phenotype namely forelimb defects to further unravel the cellular and molecular mechanisms underlying this malformation. We measured various parameters expected to be directly or indirectly influenced by irradiation damage. The mouse fetuses were irradiated at day 12 p.c. (post conception) and examined for forelimb defects on gestational days 15, 16, 17 and 19 of development. The release of inflammatory cytokines was determined in the amniotic fluid on day 16 p.c. and the mean telomere lengths assessed at days 12, 13 and 19 p.c. Differential gene expression within the forelimb bud tissues was determined using Real Time quantitative PCR (RTqPCR) 24 h following irradiation. Apoptosis was investigated in the normal and malformed fetuses using the TUNEL assay and RTqPCR. First, we found that irradiated fetuses with f...Continue Reading

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Citations

Jan 6, 2010·Radiation and Environmental Biophysics·Soile TapioMichael J Atkinson
Aug 9, 2012·Journal of Radiation Research·Nasrin BegumGuillaume Vares
Mar 17, 2010·Journal of Reproductive Immunology·Arkady Torchinsky, Vladimir Toder
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Mar 8, 2012·Annals of Plastic Surgery·Mohammad M Al-Qattan
Jan 27, 2015·Archives of Pharmacal Research·Yongjoon Suh, Su-Jae Lee
Feb 15, 2017·American Journal of Reproductive Immunology : AJRI·Elizabeth A Bonney

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