PMID: 15387151Sep 25, 2004Paper

Ionizing radiation induces a stress response in primary cultures of rainbow trout skin

Radiation Research
E M LyngC Mothersill

Abstract

Fish skin is very vulnerable to damage from physical and chemical pollutants because it is in direct contact with the aquatic environment. In this study, the effect of gamma radiation on primary cultures of rainbow trout skin was investigated. Primary cultures containing two cell types, epidermal cells and goblet mucous cells, were exposed to doses ranging from 0.5-15 Gy 60Co gamma radiation. Expression of PCNA, c-myc and BCL2 was investigated as well as growth and levels of apoptosis and necrosis. Morphological and functional changes were also studied. The irradiated cultures showed evidence of a dose-dependent increase in necrosis and enhanced proliferation as well as morphological damage. In addition, mucous cell area was found to decrease significantly after irradiation. The study shows the value of these primary cultures as in vitro models for studying radiation effects. They provide an effective alternative to whole-animal exposures for radiation risk assessment.

References

Nov 1, 1994·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L S OrtegoW H Benson
Oct 1, 1994·Current Eye Research·A P CullenJ G Sivak
Aug 30, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G EvanM Bennett
Jul 1, 1997·International Journal of Radiation Biology·J P O'Reilly, C Mothersill
Jan 13, 1998·Molecular and Cellular Endocrinology·J P Panno, B A McKeown
Mar 31, 1998·Cell Biochemistry and Function·M Lyons-AlcantaraC Mothersill
Oct 17, 1998·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·P Burkhardt-HolmW Meier
Dec 19, 2000·Biochemical and Biophysical Research Communications·M C ChenJ L Wu

❮ Previous
Next ❯

Citations

Sep 26, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Diana M LimMei-Lun Wang
May 15, 2013·Journal of Environmental Radioactivity·Carmel Mothersill, Colin Seymour
Jul 27, 2007·International Journal of Radiation Biology·Alicia O'Neill-MehlenbacherColin Seymour
Apr 13, 2007·Journal of Environmental Radioactivity·C MothersillC B Seymour
Jan 18, 2006·Mutation Research·Carmel Mothersill, C B Seymour
Oct 15, 2005·Ecotoxicology and Environmental Safety·Sharon Ní ShúilleabháinMaria Davoren
Nov 12, 2013·Brazilian Journal of Biology = Revista Brasleira De Biologia·R M S AlvesF H Caetano
May 21, 2009·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Carmel Mothersill, Colin Seymour

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis