Ionizing radiation induces epithelial-mesenchymal transition in human bronchial epithelial cells.

Bioscience Reports
Bo TangYu Tu

Abstract

The present study aimed to analyze the mechanism by which long-term occupational exposure of workers to low-dose ionizing irradiation induces epithelial-mesenchymal transition (EMT) of the human bronchial epithelial cells using transcriptome profiling. RNA-seq transcriptomics was used to determine gene expression in blood samples from radiation-exposed workers followed by bioinformatics analysis. Normal bronchial epithelial cells (16HBE) were irradiated for different durations and subjected to immunofluorescence, Western blotting, scratch healing, and adhesion assays to detect the progression of EMT and its underlying molecular mechanisms. Transcriptomics revealed that exposure to ionizing radiation led to changes in the expression of genes related to EMT, immune response, and migration. At increased cumulative doses, ionizing radiation-induced significant EMT, as evidenced by a gradual decrease in the expression of E-cadherin, increased vimentin, elevated migration ability, and decreased adhesion capability of 16HBE cells. The expression of fibronectin 1 (FN1) showed a gradual increase with the progression of EMT, and may be involved in EMT. Ionizing radiation induces EMT. FN1 may be involved in the progression of EMT and coul...Continue Reading

References

Jul 6, 2004·American Journal of Epidemiology·Shannon C BrownA James Ruttenber
Nov 26, 2008·Oncogene·G Moreno-BuenoA Cano
Apr 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Irene H HeijinkAndras Kapus
Feb 9, 2013·Radiation Research·E S GilbertN A Koshurnikova
Oct 10, 2013·Advances in Biological Regulation·Julian Gomez-Cambronero
Oct 24, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Xiao-Ming WuWen-Ru Tang
Apr 4, 2015·American Journal of Epidemiology·Alexander P KeilMelissa A Troester
Jul 3, 2015·Nature·Alison Abbott
Jul 8, 2015·International Journal of Epidemiology·Ghassan B HamraAusrele Kesminiene
Aug 8, 2015·Lancet·Kenji KamiyaRichard Wakeford
May 22, 2016·Biochemical and Biophysical Research Communications·Weiwei GaoQingqing Feng
Jul 29, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sida QinHong Ren
Oct 22, 2016·Brazilian Journal of Otorhinolaryngology·Xue JiangWeiwei Lu
Mar 28, 2017·Mutation Research. Reviews in Mutation Research·Janet HallElisabeth Cardis
Apr 5, 2017·Anticancer Research·Nikolaos TsoukalasStamatios Theocharis
Oct 7, 2017·Radiation Research·Michael GilliesElena Labutina
May 21, 2018·Biomolecular Concepts·Christina KörbelMarkus Greiner
Oct 26, 2018·Frontiers in Immunology·Carole BrosseauSophie Brouard
Mar 17, 2019·Molecular and Cellular Biochemistry·Ricardo Diaz-AragonEduardo Perez Salazar

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Methods Mentioned

BETA
PCR
X-ray
fluorescence microscopy
electrophoresis
RNA-seq

Software Mentioned

ImageJ
Hisat2
Cuffdiff
Cutadapt

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