Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint

Free Radical Biology & Medicine
Tohru YamamoriOsamu Inanami

Abstract

Whereas ionizing radiation (Ir) instantaneously causes the formation of water radiolysis products that contain some reactive oxygen species (ROS), ROS are also suggested to be released from biological sources in irradiated cells. It is now becoming clear that these ROS generated secondarily after Ir have a variety of biological roles. Although mitochondria are assumed to be responsible for this Ir-induced ROS production, it remains to be elucidated how Ir triggers it. Therefore, we conducted this study to decipher the mechanism of Ir-induced mitochondrial ROS production. In human lung carcinoma A549 cells, Ir (10 Gy of X-rays) induced a time-dependent increase in the mitochondrial ROS level. Ir also increased mitochondrial membrane potential, mitochondrial respiration, and mitochondrial ATP production, suggesting upregulation of the mitochondrial electron transport chain (ETC) function after Ir. Although we found that Ir slightly enhanced mitochondrial ETC complex II activity, the complex II inhibitor 3-nitropropionic acid failed to reduce Ir-induced mitochondrial ROS production. Meanwhile, we observed that the mitochondrial mass and mitochondrial DNA level were upregulated after Ir, indicating that Ir increased the mitochondri...Continue Reading

References

Jan 1, 1994·International Journal of Radiation Biology·P A Riley
May 1, 1997·Free Radical Research·M BattinoG Lenaz
Oct 24, 2003·Free Radical Biology & Medicine·Ramasamy P PandianPeriannan Kuppusamy
Nov 19, 2003·Archives of Biochemistry and Biophysics·Ramasamy P PandianPeriannan Kuppusamy
Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi
Mar 1, 2005·Cell·Robert S BalabanToren Finkel
Nov 22, 2005·Journal of Biochemical and Biophysical Methods·Ana GomesJosé L F C Lima
Jan 6, 2007·PloS One·Marta Martínez-DiezJosé M Cuezva
Mar 29, 2007·Radiation Research·Paula MaguireFiona M Lyng
Nov 24, 2007·Biochemical and Biophysical Research Communications·Yoshihisa TateishiTetsuya Yamamoto
Dec 14, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Hirotada FujiiHiroshi Hirata
May 22, 2008·American Journal of Physiology. Endocrinology and Metabolism·Vladimir Ljubicic, David A Hood
Oct 28, 2008·Biochemical and Biophysical Research Communications·Qing LiuHong Zhang
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kasturi MitraJennifer Lippincott-Schwartz
Sep 22, 2009·Analytical Biochemistry·Caroline DiepartBernard Gallez
Oct 7, 2009·Molecular and Cellular Endocrinology·A H V RemelsH R Gosker
Jun 11, 2010·Essays in Biochemistry·Martin JastrochMartin D Brand
Jun 15, 2010·Environmental and Molecular Mutagenesis·Michelle Barbi de MouraBennett Van Houten
Sep 22, 2010·International Journal of Radiation Biology·Sharon NugentJames E J Murphy
May 20, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cécile Cottet-RousselleJean-François Mayol
Oct 27, 2011·Free Radical Biology & Medicine·Balaraman KalyanaramanHarry Ischiropoulos

❮ Previous
Next ❯

Citations

Jul 9, 2013·Trends in Molecular Medicine·Marco DuranteKathryn D Held
Jun 15, 2013·Cell Death & Disease·A Bartoletti-StellaG Gasparre
Nov 5, 2013·Antioxidants & Redox Signaling·Aris T Alexandrou, Jian Jian Li
Dec 19, 2013·Journal of Radiation Research·Laura TeodoriDario Coletti
Oct 16, 2013·Antioxidants & Redox Signaling·Lijian ShaoDaohong Zhou
Jan 3, 2014·Antioxidants & Redox Signaling·Julie A ReiszCristina M Furdui
Jan 15, 2016·Free Radical Research·Madhuri ChaurasiaKulbhushan Sharma
May 21, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Abraham Pedroza-TorresCarlos Pérez-Plasencia
Feb 7, 2016·Seminars in Cell & Developmental Biology·Souparno Bhattacharya, Aroumougame Asaithamby
Jul 18, 2015·Environmental Research·Ryan ClantonGamal Akabani
Nov 7, 2015·Oxidative Medicine and Cellular Longevity·Li ZuoThomas M Best
Jul 16, 2015·Journal of Cellular and Molecular Medicine·Ihtzaz Ahmed MalikHendrik Andreas Wolff
Sep 30, 2015·Cellular and Molecular Bioengineering·Sandra M Baker-GrobergOwen J T McCarty
Feb 5, 2015·International Journal of Radiation Biology·Irena Szumiel
Mar 12, 2015·International Journal of Radiation Biology·Dae Yeon KimYong Weon Seo
Jul 19, 2015·Cancer Letters·Lingying TongLi Zuo
Aug 15, 2015·Oxidative Medicine and Cellular Longevity·Jiahui Liu, Zhichong Wang
Mar 8, 2013·Radiation Research·Stéphanie BlockhuysCarlos De Wagter
Jan 8, 2015·Cellular Oncology (Dordrecht)·Ronen ShavitNir Peled
Apr 29, 2015·Mutation Research·Johannes L FrießChristiane Thielemann
Apr 22, 2016·Dysphagia·Suzanne N KingTeresa Pitts
Jul 31, 2013·Free Radical Biology & Medicine·Winnie Wai-Ying Kam, Richard B Banati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.