Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells

Frontiers in Immunology
Patrick VoosGerhard Thiel

Abstract

Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was hig...Continue Reading

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Citations

Jun 28, 2019·Arhiv za higijenu rada i toksikologiju·Dauren BotbayevSabrina Angelini
May 12, 2020·Frontiers in Pharmacology·Daniela PalmeStephan M Huber
Apr 2, 2020·International Journal of Molecular Sciences·Simone MoertlMichael J Atkinson
Feb 5, 2021·Scientific Reports·Giuseppe PezzottiOsam Mazda
Jun 27, 2021·Experimental Cell Research·Tibor TeplickýBeata Čunderlíková

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Methods Mentioned

BETA
density gradient centrifugation
X-ray
flow cytometry
PCR
FACS
GTPase

Software Mentioned

Fiji
Excel
Patchmaster
CytExpert
FlowJo
MATLAB
Image Studio

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