PMID: 9555040May 23, 1998Paper

Ionophore A23187-induced leukotriene biosynthesis in equine granulocytes-neutrophils, but not eosinophils require exogenous arachidonic acid

Biochimica Et Biophysica Acta
Asa LindbergJan Ake Lindgren

Abstract

Equine granulocyte suspensions, mainly consisting of neutrophils, failed to produce detectable amounts of leukotrienes when stimulated with calcium ionophore A23187 alone, whereas leukotrienes were dose-dependently formed in control incubations with human granulocytes. In contrast, ionophore A23187 initiated synthesis of leukotrienes B4 and C4 in equine granulocytes when added in combination with low concentrations of exogenous arachidonic acid. Similarly, ionophore A23187 provoked leukotriene biosynthesis when added alone to human whole blood, whereas addition of exogenous arachidonic acid was a prerequisite for ionophore A23187-induced leukotriene formation in equine whole blood. Leukotriene biosynthesis was provoked by A23187 alone after addition of homologous platelets to equine granulocyte suspensions. After separation of equine neutrophils and eosinophils, purified eosinophil suspensions produced LTC4 after stimulation with ionophore A23187 alone, whereas exogenous arachidonic acid was required for ionophore-induced LTB4 formation in purified neutrophil suspensions. Leukotriene synthesis in both eosinophils and neutrophils was suppressed by the 5-lipoxygenase activating protein (FLAP) inhibitor, MK-886. Exogenous arachido...Continue Reading

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Citations

May 20, 2008·Journal of Animal Physiology and Animal Nutrition·B FuchsJ Schiller
Jan 18, 2018·Cell and Tissue Research·Stacy L Anderson, Baljit Singh
Mar 19, 2004·American Journal of Veterinary Research·Asa LindbergJan Ake Lindgren
Jun 14, 2002·American Journal of Veterinary Research·Asa LindbergN Edward Robinson
Feb 5, 2000·Equine Veterinary Journal. Supplement·M Jensen-WaernB Essén-Gustavsson

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