Ionophore A23187: the effect of H+ concentration on complex formation with divalent and monovalent cations and the demonstration of K+ transport in mitochondria mediated by A23187

Biochemistry
D R Pfeiffer, H A Lardy

Abstract

The two-phase extraction technique has been used to study the equilibrium between A23187, metal cations, and H+. Under these conditions the ionophore forms charge neutral isostoichiometric complexes with divalent cations in which both carboxylate groups of the 2:1 A23187:M2+ complexes are deprotonated. In ethanol, however, the methyl ester of A23187 also binds divalent cations indicating that protonated complexes between A23187 and cations should also exist. With monovalent cations, A23187 forms two charge-neutral complexes of stoichiometries and relative stabilities: A2HM greater than AM. Examination of energy utilization K+ and H+ movements, and light scattering capacity of mitochondria in the presence of divalent cation chelators, A23187, and valinomycin demonstrates that A23187 can act as a nigericin type K+ ionophore under appropriate conditions. Formation constants for the A2HM complexes with monovalent cations indicate that with appropriate conditions transport of Li+ and Na+ mediated by A23187 would also be expected. The binding constant data and associated free energies of complex formation are compared as a function of ionic radius and of cation charge. The data indicate that lack of conformational mobility in A23187 ...Continue Reading

References

Apr 21, 1975·Biochimica Et Biophysica Acta·A HyonoS L Bonting
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·Z SelingerM Schramm
Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·B C PressmanJ H Johnson
Mar 20, 1974·Journal of the American Chemical Society·M O ChaneyJ L Occolowitz
Jan 1, 1971·Annual Review of Microbiology·P J Henderson
Oct 6, 1972·Biochemical and Biophysical Research Communications·A H Caswell, B C Pressman
Oct 31, 1969·Annals of the New York Academy of Sciences·B C Pressman

Citations

Jan 1, 1979·Planta·T H Chen, L F Jaffe
Dec 1, 1987·Archives of Toxicology·L Pezzi, W Y Cheung
Aug 1, 1980·Journal of Bioenergetics and Biomembranes·G Y ShiG P Brierley
Jan 1, 1989·Neurochemical Research·R A PhilibertG R Dutton
Mar 8, 1977·The Journal of Membrane Biology·E F LaBelle, E Racker
Aug 15, 1977·Experientia·W J MalaisseG Somers
Oct 15, 1982·Experientia·K S Pyant, G P Brierley
Jul 1, 1991·The Journal of Membrane Biology·T J Simons
Jan 1, 1981·Archives of Biochemistry and Biophysics·J S PuskinM T Coene
Sep 19, 1977·Biochimica Et Biophysica Acta·S P Young, B D Gomperts
Jun 11, 1990·Biochimica Et Biophysica Acta·J A Veiro, P R Cullis
Aug 1, 1980·Cellular Immunology·J D JohnsonN L King-Thompson
Jan 1, 1986·Biochimica Et Biophysica Acta·K D Garlid, A D Beavis
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N Kaiser, I S Edelman
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J MoranJ A Fischer
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G WeissmannE Goodman
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M DeleersW J Malaisse
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R BrasseurJ M Ruysschaert
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R Brasseur, M Deleers
Jan 22, 2010·The Journal of Biological Chemistry·Florentina PenaIneke Braakman
Feb 1, 1978·The Journal of Cell Biology·J C GilkeyG T Reynolds
Sep 1, 1987·The Journal of Experimental Medicine·H Takayama, M V Sitkovsky
Feb 1, 1987·The Journal of General Physiology·G D NicolM D Bownds
Mar 1, 1987·The Journal of General Physiology·P P Schnetkamp, M D Bownds
Dec 1, 1979·Antimicrobial Agents and Chemotherapy·B J AbbottL Farhner
Dec 1, 1976·The Journal of Clinical Investigation·J L IveyA H Tashjian
Aug 17, 1978·Biochimica Et Biophysica Acta·M Hershkowitz
Jan 1, 1993·Critical Reviews in Microbiology·J Youatt
Aug 18, 2004·Archives of Biochemistry and Biophysics·Majidreza KamyarRosa Lemmens-Gruber
Mar 17, 1977·Biochimica Et Biophysica Acta·J Wulf, W G Pohl
Jan 15, 1983·Thrombosis Research·B Lages, C Kruger
Jun 1, 1985·Cell Calcium·T R Hinds, F F Vincenzi
May 1, 1982·Cell Calcium·U B Kaupp, P P Schnetkamp
May 1, 1984·British Journal of Pharmacology·E J BarbieriR F McMichael
Jan 24, 1977·Biochemical and Biophysical Research Communications·J Duszyński, L Wojtczak
Jul 1, 1980·Archives of Biochemistry and Biophysics·G Ben-Hayyim, G H Krause
Jan 8, 1981·Biochimica Et Biophysica Acta·J T O'FlahertyC E McCall
Mar 2, 1999·Archives of Biochemistry and Biophysics·J WudarczykE Lenartowicz
May 17, 1977·Biochemistry·T C Wun, R Bittman
Nov 23, 1982·Biochemistry·J R White, F L Pearce
Jan 29, 1985·Biochemistry·P P Schnetkamp, U B Kaupp
Aug 11, 1987·Biochemistry·M B SankaramK R Easwaran
Sep 3, 1991·Biochemistry·P ZimniakR Lester
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd

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Calcimycin
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