Ionotropic and metabotropic activation of a neuronal chloride channel by serotonin and dopamine in the leech Hirudo medicinalis

The Journal of Physiology
D W AliP Drapeau

Abstract

1. Cl- channels on the pressure-sensitive (P) neuron in the leech are directly activated by synaptic release of serotonin (5-HT) and are indirectly stimulated by the cAMP second messenger pathway, suggesting an unusual dual regulation of the channels. We have investigated the mode of action of 5-HT and dopamine (DA) on a Cl- channel in adult P cells in culture by recording from cell-attached patches. 2. 5-HT increased Cl- channel activity only when included in the recording pipette and not when applied in the bath. 3. Pipette or, more effectively, bath application of DA led to an increase in Cl- channel activity. This effect was blocked by the potent and specific dopaminergic (DA1) receptor blocker, SCH-23390. 4. The stimulation by DA, but not by 5-HT, was also blocked by the cAMP-dependent protein kinase A (PKA) inhibitor Rp-cAMP and was mimicked by the membrane-permeant cAMP analogue dibutyryl cAMP (db-cAMP). 5. Our results show that 5-HT directly gates a Cl- channel that is also activated by DA via the cAMP pathway. This study demonstrates that a ligand-gated channel can be independently operated by another transmitter acting via a second messenger pathway.

References

Mar 1, 1977·Journal of Neurophysiology·P B SargentJ G Nicholls
Jan 1, 1988·Methods in Enzymology·L H BotelhoB Jastorff
Sep 9, 1986·European Journal of Pharmacology·A SidhuJ W Kebabian
Jan 1, 1974·Cell and Tissue Research·A E StuartZ W Hall
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1993·Journal of Neurochemistry·S J Peroutka
Jan 1, 1994·Molecular Endocrinology·J D Scott, S McCartney
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y T WangM W Salter
May 1, 1996·Pflügers Archiv : European journal of physiology·M GlitschA Karschin

❮ Previous
Next ❯

Citations

Jul 22, 2004·Behavioural Brain Research·Maria Luisa ZaccardiMarcello Brunelli
Jan 1, 2007·The Libyan Journal of Medicine·Sm AburawiZm Gorash
Jun 17, 1998·The Journal of Physiology·N S Magoski, L K Kaczmarek
Sep 29, 2004·Journal of Neurobiology·Paul Wilhelm Dierkes, Wolf-Rüdiger Schlue
Oct 6, 2009·Nature Neuroscience·Quentin Gaudry, William B Kristan
Sep 28, 2002·Nature Neuroscience·Susan L IngramSusan G Amara
Nov 9, 2002·Journal of Neurophysiology·Rossana ScuriMarcello Brunelli
Mar 18, 2011·Journal of Neurophysiology·Giacomo Bisson, Vincent Torre
May 16, 2001·Journal of Neurophysiology·K A MesceL S Gilchrist
Jun 9, 2004·The Journal of Experimental Biology·S T MukaiA S M Saleuddin
Jun 5, 2012·The Journal of Experimental Biology·Kevin M CrispKaren A Mesce
Nov 8, 2002·Biochimica Et Biophysica Acta·Mikael SchnizlerWolfgang Clauss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.