IP3 receptor in the hair cells of frog semicircular canal and its possible functional role

The European Journal of Neuroscience
M L RossiRiccardo Fesce

Abstract

The presence and functional role of inositol trisphosphate receptors (IP3R) was investigated by electrophysiology and immunohistochemistry in hair cells from the frog semicircular canal. Intracellular recordings were performed from single fibres of the posterior canal in the isolated, intact frog labyrinth, at rest and during rotation, in the presence of IP3 receptor inhibitors and drugs known to produce Ca2+ release from the internal stores or to increase IP3 production. Hair cell immunolabelling for IP3 receptor was performed by standard procedures. The drug 2-aminoethoxydiphenyl borate (2APB), an IP3 receptor inhibitor, produced a marked decrease of mEPSP and spike frequency at low concentration (0.1 mm), without affecting mEPSP size or time course. At high concentration (1 mm), 2APB is reported to block the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA pump) and increase [Ca2+]i; at the labyrinthine cytoneural junction, it greatly enhanced the resting and mechanically evoked sensory discharge frequency. The selective agonist of group I metabotropic glutamate receptors (RS)-3,5-dihydroxyphenylglycine (DHPG, 0.6 mm), produced a transient increase in resting mEPSP and spike frequency at the cytoneural junction, with no...Continue Reading

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Citations

Dec 12, 2012·American Journal of Physiology. Renal Physiology·M Cecilia Ortiz-CapisanoWilliam H Beierwaltes
Feb 10, 2012·Journal of Biomedicine & Biotechnology·Paola PerinUmberto Laforenza
Jun 28, 2014·Frontiers in Cellular Neuroscience·Manuel Castellano-Muñoz, Anthony J Ricci
Dec 4, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Maria Lisa RossiRiccardo Fesce
Nov 20, 2009·Acta Oto-laryngologica·Masaya Takumida, Matti Anniko
Oct 30, 2015·Journal of Neurophysiology·Manuel Castellano-MuñozAnthony J Ricci

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