IQGAP1, AmotL2, and FKBP51 Scaffoldins in the Glioblastoma Microenvironment

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Deborah RotoliPablo Martín-Vasallo

Abstract

Glioblastoma (GB) is the most frequently occurring and aggressive primary brain tumor. Glioma stem cells (GSCs) and astrocytoma cells are the predominant malignant cells occurring in GB besides a highly heterogeneous population of migrating, neovascularizing and infiltrating myeloid cells that forms a complex tumor microenvironment (TME). Cross talk between the TME cells is pivotal in the biology of this tumor and, consequently, adaptor proteins at critical junctions of signaling pathways may be crucial. Scaffold proteins (scaffolins or scaffoldins) integrate external and internal stimuli to regulate various signaling pathways, interacting simultaneously with multiple proteins involved. We investigated by double and triple immunofluorescence the localization of IQGAP1, AmotL2, and FKBP51, three closely related scaffoldins, in malignant cells and TME of human GB tumors. We found that IQGAP1 is preferentially expressed in astrocytoma cells, AmotL2 in GSCs, and FKBP51 in white blood cells in human GB tumors. As GSCs are specially the target for novel therapies, we will investigate in further studies whether AmotL2 inhibition is effective in the treatment of GB.

References

Oct 1, 1971·Journal of Neuropathology and Experimental Neurology·R N BakerS P Frommes
Jul 1, 1981·Cell·R Levenson, D Housman
Sep 26, 1997·The Journal of Biological Chemistry·J W EricksonM J Hart
Jun 8, 2002·EMBO Reports·Peter E Shaw
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Sep 2, 2005·Molecular and Cellular Biology·Monideepa RoyDavid B Sacks
Jun 2, 2006·Science's STKE : Signal Transduction Knowledge Environment·Joseph A Garcia
Nov 1, 2006·Cellular and Molecular Life Sciences : CMLS·S Barik
Jan 16, 2007·Cancer Cell·Christopher CalabreseRichard J Gilbertson
Mar 10, 2009·Cellular Signalling·Michael JohnsonBeric R Henderson
May 13, 2009·FEBS Letters·Colin D WhiteDavid B Sacks
Apr 28, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sayuri SuzukiHideyuki Okano
Oct 5, 2010·The FEBS Journal·László Buday, Péter Tompa
Dec 2, 2010·British Journal of Cancer·L LiL Wang
Sep 6, 2011·Trends in Endocrinology and Metabolism : TEM·Cheryl L StorerMarc B Cox
Dec 21, 2011·Cellular Signalling·Colin D WhiteDavid B Sacks
Feb 22, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Subramaniam MalarkannanMonica S Thakar
Aug 30, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yawei LiuShohreh Issazadeh-Navikas
Jan 28, 2014·Clinical and Translational Medicine·Simona RomanoMaria Fiammetta Romano
Aug 2, 2014·Nature Communications·Mahdi MojallalLars Holmgren
Aug 15, 2014·Molecular Biology of the Cell·Damien Garbett, Anthony Bretscher
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Nov 18, 2014·Biochemical Society Transactions·Anna Dimberg
Dec 17, 2014·Current Pharmaceutical Design·Zhongyong WangSuyun Huang
Mar 4, 2015·Molecular Immunology·Alex M AbelSubramaniam Malarkannan
Mar 27, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Vashendriya V V HiraCornelis J F Van Noorden
Jun 26, 2015·Genes & Development·Justin D LathiaJeremy N Rich
Jul 5, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Theo Rein
Jul 22, 2016·The Journal of Biological Chemistry·Samar SayedyahosseinDavid B Sacks

❮ Previous
Next ❯

Citations

Jun 25, 2021·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Deborah RotoliPablo Martín-Vasallo
Oct 30, 2021·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Cornelis J F van NoordenVashendriya V V Hira

❮ Previous
Next ❯

Datasets Mentioned

BETA
GMT

Methods Mentioned

BETA
GTPase
confocal microscopy

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.