Iridium Nanoparticles for Multichannel Luminescence Lifetime Imaging, Mapping Localization in Live Cancer Cells

Journal of the American Chemical Society
Siobhan M KingZoe Pikramenou

Abstract

The development of long-lived luminescent nanoparticles for lifetime imaging is of wide interest as luminescence lifetime is environmentally sensitive detection independent of probe concentration. We report novel iridium-coated gold nanoparticles as probes for multiphoton lifetime imaging with characteristic long luminescent lifetimes based on iridium luminescence in the range of hundreds of nanoseconds and a short signal on the scale of picoseconds based on gold allowing multichannel detection. The tailor-made IrC6 complex forms stable, water-soluble gold nanoparticles (AuNPs) of 13, 25, and 100 nm, bearing 1400, 3200, and 22 000 IrC6 complexes per AuNP, respectively. The sensitivity of the iridium signal on the environment of the cell is evidenced with an observed variation of lifetimes. Clusters of iridium nanoparticles show lifetimes from 450 to 590 ns while lifetimes of 660 and 740 ns are an average of different points in the cytoplasm and nucleus. Independent luminescence lifetime studies of the nanoparticles in different media and under aggregation conditions postulate that the unusual long lifetimes observed can be attributed to interaction with proteins rather than nanoparticle aggregation. Total internal reflection fl...Continue Reading

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Citations

Jan 19, 2019·Nanoscale·Jun YangXuesong Mei
Nov 15, 2019·Chemical Communications : Chem Comm·Yutong HouShiping Yang
Mar 10, 2020·Chemical Society Reviews·Cristóbal QuintanaMark G Humphrey
Oct 17, 2019·Dalton Transactions : an International Journal of Inorganic Chemistry·Orbett T AlexanderHendrik G Visser
Nov 24, 2021·Chemistry : a European Journal·Andrew J CarrodZoe Pikramenou

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