Irinotecan induces enterocyte cell death and changes to muc2 and muc4 composition during mucositis in a tumour-bearing DA rat model

Cancer Chemotherapy and Pharmacology
Daniel ThorpeAndrea M Stringer

Abstract

Irinotecan-induced mucositis is a major oncological problem. Goblet cells secrete mucus, protecting the intestinal mucosa, with secretion altered during mucositis. The enteric nervous system is involved in regulating gut motility and secretion. The aim of this study was to determine whether enteric neural cells and goblet cells are altered following irinotecan treatment. Tumour-bearing Dark Agouti rats were administered a single dose of 175 mg/kg of irinotecan intraperitoneally and 0.01 mg/kg atropine subcutaneously. Experimental and untreated control rats were killed at times 6, 24, 48, 72, 96 and 120 h after treatment. Jejunum and colon samples were formalin fixed. Haematoxylin and eosin staining, Alcian Blue-PAS staining, and immunohistochemistry with S-100 antibody (neural cell marker) were carried out. Statistical analyses were carried out using Kruskal-Wallis test with Dunns post test, Mann Whitney U test, and nonlinear regression. Total goblet cells decreased at 72 h compared with controls in the colon (p < 0.05). The percentage of cavitated goblet cells decreased compared to all other time points at 120 h in the colon. The number of S-100-positive cells in the submucosal plexus decreased in the colon (p = 0.0046) and in...Continue Reading

References

Dec 11, 1992·Oral Surgery, Oral Medicine, and Oral Pathology·S T SonisM Nicolson
Feb 1, 1991·The American Journal of Physiology·R D Specian, M G Oliver
May 1, 1988·JPEN. Journal of Parenteral and Enteral Nutrition·K A Kern, J A Norton
Jan 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L SaltzD Khayat
Apr 1, 1997·Clinical Science·D M KeefeR E Sage
May 1, 1997·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·A M Robertson, D P Wright
Aug 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D M SavareseF M Stewart
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Sep 17, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S WadlerE Vokes
Oct 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M VerburgJ Dekker
Jun 18, 2002·Journal of Cellular and Molecular Medicine·J R Jass, M D Walsh
Jul 10, 2002·Progress in Nucleic Acid Research and Molecular Biology·Kermit L CarrawayCoralie A Carothers Carraway
Dec 25, 2002·Digestive Diseases and Sciences·Rachel J GibsonAdrian G Cummins
Nov 8, 2003·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Dorothy M K Keefe
Apr 27, 2004·Cancer·Stephen T SonisUNKNOWN International Society for Oral Oncology
Sep 14, 2004·The Biochemical Journal·Catherine RobbeJean-Claude Michalski
Apr 1, 2005·International Journal of Cancer. Journal International Du Cancer·Rachel J GibsonDorothy M K Keefe
Jun 27, 2006·Cancer Chemotherapy and Pharmacology·Joanne M BowenDorothy M K Keefe
Sep 26, 2006·Journal of Cellular Physiology·Barbara A E de KoningIngrid B Renes
Jan 20, 2007·Cancer·Dorothy M KeefeUNKNOWN Mucositis Study Section of the Multinational Association of Supportive Care in Cancer and the International Society for Oral
Apr 3, 2007·International Journal of Radiation Oncology, Biology, Physics·Linda S EltingAdam S Garden
Aug 24, 2007·Chemotherapy·Rachel J GibsonDorothy M K Keefe
Mar 21, 2008·Cancer Chemotherapy and Pharmacology·Richard M LoganDorothy M K Keefe
Apr 17, 2008·Scandinavian Journal of Gastroenterology·Yoichi SaegusaKazuhiko Ishihara
Jun 7, 2008·Cancer Biology & Therapy·Richard M LoganDorothy M K Keefe
Oct 18, 2008·Cancer Biology & Therapy·Andrea M StringerDorothy M K Keefe
Oct 22, 2008·Scandinavian Journal of Gastroenterology·Yoichi SaegusaKazuhiko Ishihara
Nov 11, 2008·Cancer Chemotherapy and Pharmacology·Andrea M StringerDorothy M K Keefe
Sep 22, 2009·International Journal of Experimental Pathology·Andrea M StringerDorothy M K Keefe
Apr 17, 2015·World Journal of Surgical Oncology·Alessandro SanguinettiNicola Avenia

❮ Previous
Next ❯

Citations

Aug 16, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Christian Bailly

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved