Iris as a recipient tissue for pigment cells: organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Hitomi AokiTakahiro Kunisada

Abstract

Regenerative transplantation of embryonic stem (ES) cell-derived melanocytes into adult tissues, especially skin that includes hair follicles or the hair follicle itself, generally not possible, whereas that of ES cell-derived pigmented epithelium was reported previously. We investigated the in vivo differentiation of these two pigment cell types derived from ES cells after their transfer into the iris. Melanocytes derived from ES cells efficiently integrated into the iris and expanded to fill the stromal layer of the iris, like those prepared from neonatal skin. Transplanted pigmented epithelium from either ES cells or the neonatal eye was also found to be integrated into the iris. Both types of these regenerated pigment cells showed the correct morphology. Regenerated pigment epithelium expressed its functional marker. Functional blocking of signals required for melanocyte development abolished the differentiation of transplanted melanocytes. These results indicate successful in vivo regenerative transfer of pigment cells induced from ES cells in vitro.

References

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Jul 10, 2004·Journal of the American Academy of Dermatology·Yu-Fu ChenChih-Ming Hung
Mar 16, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hitomi AokiTakahiro Kunisada
May 3, 2006·The Journal of Cell Biology·Mariko MoriyamaShin-Ichi Nishikawa
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Oct 14, 2006·Stem Cells·Tsutomu MotohashiTakahiro Kunisada
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Nov 16, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Hitomi AokiTakahiro Kunisada
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Feb 28, 2008·Transplantation·Hitomi AokiTakahiro Kunisada

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Citations

Jun 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hitomi AokiTakahiro Kunisada
Jun 17, 2014·Journal of Korean Medical Science·Seok Hyun LeeJae Chan Kim
Nov 9, 2010·Pigment Cell & Melanoma Research·Hitomi AokiTakahiro Kunisada

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