Iron- and Hepcidin-Independent Downregulation of the Iron Exporter Ferroportin in Macrophages during Salmonella Infection

Frontiers in Immunology
Alexandra WillemetzFrançois Canonne-Hergaux

Abstract

Retention of iron in tissue macrophages via upregulation of hepcidin (HAMP) and downregulation of the iron exporter ferroportin (FPN) is thought to participate in the establishment of anemia of inflammation after infection. However, an upregulation of FPN has been proposed to limit macrophages iron access to intracellular pathogens. Therefore, we studied the iron homeostasis and in particular the regulation of FPN after infection with Salmonella enterica serovar Typhimurium in mice presenting tissue macrophages with high iron (AcB61), basal iron (A/J and wild-type mice), or low iron (Hamp knock out, Hamp(-/-)) levels. The presence of iron in AcB61 macrophages due to extravascular hemolysis and strong erythrophagocytosis activity favored the proliferation of Salmonella in the spleen and liver with a concomitant decrease of FPN protein expression. Despite systemic iron overload, no or slight increase in Salmonella burden was observed in Hamp(-/-) mice compared to controls. Importantly, FPN expression at both mRNA and protein levels was strongly decreased during Salmonella infection in Hamp(-/-) mice. The repression of Fpn mRNA was also observed in Salmonella-infected cultured macrophages. In addition, the downregulation of FPN wa...Continue Reading

References

Mar 21, 1998·Journal of Gastroenterology and Hepatology·J L PlummerP de la M Hall
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C M RosenbergerB B Finlay
Aug 6, 2002·The Journal of Biological Chemistry·Funmei YangDavid J Haile
Nov 5, 2003·Nature Genetics·Gundula Min-OoPhilippe Gros
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jiying SunKazuhiko Igarashi
Aug 6, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·François Canonne-HergauxPhilippe Gros
Aug 13, 2005·Experimental Cell Research·Constance DelabyFrançois Canonne-Hergaux
Apr 1, 2006·Blood·Jeanne-Claire Lesbordes-BrionSophie Vaulont
Apr 20, 2006·Infection and Immunity·Sabine ChlostaBobby J Cherayil
Nov 14, 2007·The Journal of Experimental Medicine·Marie-France RoyDanielle Malo
Sep 30, 2009·Seminars in Hematology·Tomas Ganz, Elizabeta Nemeth
Oct 19, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy JacksonStephen J McSorley
Nov 10, 2010·PLoS Pathogens·Tania Di PietrantonioErwin Schurr
Nov 22, 2011·The Lancet Infectious Diseases·Laura J V Piddock
Jul 17, 2012·Nature Reviews. Microbiology·M Indriati Hood, Eric P Skaar
May 3, 2013·PloS One·Jean-Christophe Deschemin, Sophie Vaulont
May 21, 2013·Cell Host & Microbe·James E Cassat, Eric P Skaar
Jul 30, 2014·Hematology/oncology Clinics of North America·Elizabeta Nemeth, Tomas Ganz
Aug 1, 2014·Frontiers in Pharmacology·Manfred NairzGünter Weiss
Dec 11, 2014·MBio·M Carolina PilonietaCorrella S Detweiler
Feb 11, 2015·Blood·Claudia GuidaMartina U Muckenthaler
Jan 1, 2014·Journal of Immunology Research·Susu M ZughaierNael A McCarty
Jul 17, 2015·Bioscience Reports·Gautam RishiV Nathan Subramaniam
Jul 21, 2015·Cell Host & Microbe·Manfred NairzBruno Galy
Oct 16, 2015·Infection and Immunity·Erin M McDonaldCorrella S Detweiler

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Citations

Nov 18, 2017·International Journal of Hematology·Tomas Ganz
Feb 3, 2018·Journal of Neuroinflammation·Anne M NixonJames R Connor
Oct 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sidi YangRui Huang
Sep 12, 2018·Pharmaceuticals·Ana Cordeiro GomesMaria Salomé Gomes
May 12, 2020·World Journal of Gastroenterology : WJG·Elodie FalletEdouard Bardou-Jacquet
Jun 20, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriel NúñezMiguel P Soares
Jan 11, 2020·Haematologica·João V NevesPedro N S Rodrigues
Apr 22, 2021·Open Forum Infectious Diseases·Kjersti OppenLars Heggelund

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Methods Mentioned

BETA
PCR

Software Mentioned

Zeiss LSM Image Browser
GraphPad Prism
GraphPad

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