Iron accumulation in the choroid plexus, ependymal cells and CNS parenchyma in a rat strain with low-grade haemolysis of fragile macrocytic red blood cells.

Brain Pathology
Isabella WimmerSimon Hametner

Abstract

Iron accumulation in the CNS is associated with many neurological diseases via amplification of inflammation and neurodegeneration. However, experimental studies on iron overload are challenging, since rodents hardly accumulate brain iron in contrast to humans. Here, we studied LEWzizi rats, which present with elevated CNS iron loads, aiming to characterise choroid plexus, ependymal, CSF and CNS parenchymal iron loads in conjunction with altered blood iron parameters and, thus, signifying non-classical entry sites for iron into the CNS. Non-haem iron in formalin-fixed paraffin-embedded tissue was detected via DAB-enhanced Turnbull Blue stainings. CSF iron levels were determined via atomic absorption spectroscopy. Ferroportin and aquaporin-1 expression was visualised using immunohistochemistry. The analysis of red blood cell indices and serum/plasma parameters was based on automated measurements; the fragility of red blood cells was manually determined by the osmotic challenge. Compared with wild-type animals, LEWzizi rats showed strongly increased iron accumulation in choroid plexus epithelial cells as well as in ependymal cells of the ventricle lining. Concurrently, red blood cell macrocytosis, low-grade haemolysis and signifi...Continue Reading

References

Mar 1, 1991·Journal of Neurochemistry·E SoficM B Youdim
Feb 1, 1990·The American Journal of Physiology·M A MaktabiF M Faraci
Oct 1, 1989·The American Journal of Clinical Nutrition·S C CunnaneD F Horrobin
Jan 1, 1968·Acta Neurologica Scandinavica·J Prineas
Jun 3, 1967·British Medical Journal·E A CasparyE J Field
Mar 1, 1980·Neurology·C L SchaufF A Davis
Aug 1, 1995·The European Journal of Neuroscience·F Marín, L Puelles
May 20, 1998·The American Journal of Clinical Nutrition·Z L HarrisJ D Gitlin
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z L HarrisJ D Gitlin
Aug 30, 2000·Toxicology·J P Kehrer
Nov 23, 2000·Neurology·R KiraT Hara
Jan 3, 2001·Microscopy Research and Technique·K M DziegielewskaN R Saunders
Sep 29, 2001·The European Journal of Neuroscience·J M SerotG C Faure
Mar 7, 2003·Pediatric Research·Asha Jyothi M SiddappaMichael K Georgieff
Oct 1, 1958·Journal of Neurochemistry·B HALLGREN, P SOURANDER
Nov 10, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kotaro OshioGeoffrey T Manley
May 25, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Sue C EngKris V Kowdley
Sep 16, 2005·The New England Journal of Medicine·Deborah Rund, Eliezer Rachmilewitz
Jul 1, 1947·The Journal of Clinical Investigation·A K ParpartA M Chase
Sep 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Suh Young Jeong, Samuel David
Nov 15, 2006·Seminars in Pediatric Neurology·Betsy Lozoff, Michael K Georgieff
Jun 15, 2007·Archives of Histology and Cytology·Reiko MeguroKazuhiko Shoumura
Oct 24, 2007·Journal of Neurochemistry·Torben MoosEvan H Morgan
Mar 18, 2008·Current Opinion in Chemical Biology·Kevin J Barnham, Ashley I Bush
Oct 7, 2008·Glia·Bozho TodorichJames R Connor
Jul 15, 2009·Acta Neuropathologica·Ester Perez-GraciaIsidro Ferrer
Oct 24, 2009·Metabolic Brain Disease·Tracey A RouaultSuh Young Jeong
Jan 1, 1989·Neurochemistry International·Y KidaK Yamanouchi
Sep 3, 2010·Journal of Neural Transmission·Robert R CrichtonRoberta J Ward
Mar 15, 2011·Brain : a Journal of Neurology·James R ConnorChristopher J Earley
Feb 26, 2013·Nature Reviews. Immunology·Ravid ShechterMichal Schwartz
Jul 23, 2013·Annals of Neurology·Simon HametnerHans Lassmann
Nov 28, 2013·The Journal of Biological Chemistry·Anders EtzerodtSøren Kragh Moestrup
Dec 17, 2014·Brain Research·Jeanelle ArizaVerónica Martínez-Cerdeño
Dec 19, 2014·Methods in Molecular Biology·Jan Bauer, Hans Lassmann
Mar 27, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Melody P LunMaria K Lehtinen
Aug 8, 2015·Experimental Neurology·Reynold SpectorConrad E Johanson

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