Iron alters Ca2+ homeostasis in doxorubicin-resistant K562 cells.

Clinical and Experimental Pharmacology & Physiology
Leman YalcintepeBahire Kucukkaya

Abstract

Iron is an essential trace element especially in cell proliferation, and growth for various cellular events. An increasing amount of research has shown that iron metabolism is altered in tumour cells which usually have rapid growth rates. However, the number of studies on iron metabolism, and calcium regulation are limited in drug-resistant tumour cells. Previously, we have shown that modulation of iron metabolism through iron chelation regulated the intracellular calcium, and increased the doxorubicin sensitivity. In the present study, we investigated the effects of iron on mRNA expression profiles of fifteen key genes (IP3 R1/2/3, RYR1/2, SERCA1/2/3, NCX1/2/3, PMCA1/2/3, and PMCA4) related to calcium homeostasis in the parental cell line K562 and its subclone doxorubicin-resistant K562 cells. According to the ΔΔCt method with a two-fold expression difference (P < .05) as a cut-off level, although iron showed differential effects on most of the genes, IP3 R and PMCA genes were especially determined to have changed significantly. These results show that iron metabolism is an important metabolism due to changes in the expression of genes involved in calcium regulation and is a new perspective to overcome cancer/drug resistance.

References

Aug 1, 1976·British Journal of Haematology·A V HoffbrandM H Tattersall
Jan 1, 1989·Annual Review of Physiology·C van Breemen, K Saida
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M Simon, M Schindler
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S SimonM Schindler
Jun 1, 1997·Molecular and Cellular Biology·T Jayaraman, A R Marks
Oct 18, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·R Krishna, L D Mayer
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Mar 21, 2002·Nature Reviews. Cancer·Michael M GottesmanSusan E Bates
Apr 8, 2003·Current Medicinal Chemistry·Joan L BussSuzy V Torti
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Sten OrreniusPierluigi Nicotera
Apr 7, 2004·British Journal of Pharmacology·Shanthala PadarRoshanak Rahimian
Jan 11, 2005·The Journal of Pathology·D B Longley, P G Johnston
Jul 5, 2005·Free Radical Biology & Medicine·Eileen M HokeEmily Shacter
Aug 16, 2005·Biochemical and Biophysical Research Communications·Zdena SulováAlbert Breier
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Megan WhitnallDes R Richardson
Jan 20, 2007·The Biochemical Journal·Margarita TenopoulouUlf T Brunk
Apr 13, 2007·Physiological Reviews·J Kevin FoskettDon-On Daniel Mak
Sep 19, 2007·The Journal of Biological Chemistry·M Tariq KhanSuresh K Joseph
Mar 28, 2008·The Journal of Biological Chemistry·Camia SteinmannDarren Boehning
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Mar 30, 2012·Current Medicinal Chemistry·Y YuD R Richardson
Apr 19, 2013·Nature Reviews. Cancer·Suzy V Torti, Frank M Torti
Sep 23, 2014·The Journal of Toxicological Sciences·Nawaf Al-TaweelDietrich Büsselberg
Dec 17, 2014·Biochimica Et Biophysica Acta·Tamara VervloessemJan B Parys
Nov 20, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Lisann PelzlFlorian Lang
Dec 1, 2015·The Journal of Clinical Investigation·Matthew L HedbergJennifer R Grandis
Jul 7, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Lisann PelzlFlorian Lang

❮ Previous
Next ❯

Citations

Jun 8, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Yuhua LeiYan-Zhong Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.