PMID: 11335103May 4, 2001Paper

Iron and gallium increase iron uptake from transferrin by human melanoma cells: further examination of the ferric ammonium citrate-activated iron uptake process

Biochimica Et Biophysica Acta
D R Richardson

Abstract

Previously we showed that preincubation of cells with ferric ammonium citrate (FAC) resulted in a marked increase in Fe uptake from both (59)Fe-transferrin (Tf) and (59)Fe-citrate (D.R. Richardson, E. Baker, J. Biol. Chem. 267 (1992) 13972-13979; D.R. Richardson, P. Ponka, Biochim. Biophys. Acta 1269 (1995) 105-114). This Fe uptake process was independent of the transferrin receptor and appeared to be activated by free radicals generated via the iron-catalysed Haber-Weiss reaction. To further understand this process, the present investigation was performed. In these experiments, cells were preincubated for 3 h at 37 degrees C with FAC or metal ion solutions and then labelled for 3 h at 37 degrees C with (59)Fe-Tf. Exposure of cells to FAC resulted in Fe uptake from (59)Fe-citrate that became saturated at an Fe concentration of 2.5 microM, while FAC-activated Fe uptake from Tf was not saturable up to 25 microM. In addition, the extent of FAC-activated Fe uptake from citrate was far greater than that from Tf. These results suggest a mechanism where FAC-activated Fe uptake from citrate may result from direct interaction with the transporter, while Fe uptake from Tf appears indirect and less efficient. Preincubation of cells with F...Continue Reading

References

Jun 7, 1991·Biochimica Et Biophysica Acta·D Richardson, E Baker
Jun 12, 1990·Biochimica Et Biophysica Acta·D R Richardson, E Baker
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D TrinderE Baker
Sep 30, 1986·Biochemical and Biophysical Research Communications·H LöwD J Morre
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw
Jan 18, 1983·Biochemistry·W R Harris, V L Pecoraro
Oct 1, 1984·Journal of Cellular Physiology·M TiteuxJ Breton-Gorius
Jun 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D TrinderP Aisen
Jan 31, 1997·The Journal of Biological Chemistry·O OlakanmiB E Britigan
Mar 14, 1997·Biochimica Et Biophysica Acta·D R Richardson, P Ponka
Dec 31, 1997·The Journal of Cell Biology·J A GutierrezM Wessling-Resnick
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·M D FlemingN C Andrews
Jul 20, 1999·The Journal of Biological Chemistry·H KawabataH P Koeffler
Apr 5, 2000·The Journal of Biological Chemistry·H KawabataH P Koeffler
May 16, 2000·The Journal of Biological Chemistry·D E BarañanoC D Ferris
Dec 1, 1963·Analytical Biochemistry·M AVRON, N SHAVIT

❮ Previous
Next ❯

Citations

Jan 27, 2009·Insect Biochemistry and Molecular Biology·Guoli ZhouJoy J Winzerling
Oct 1, 2005·Chembiochem : a European Journal of Chemical Biology·Isam KhalailaPaul J Dyson
Jun 1, 2005·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Oyebode OlakanmiBradley E Britigan
Apr 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Melanie PribiskoPunnajit Lim
Mar 22, 2005·Biochimica Et Biophysica Acta·Rebecca J HendersonJames R Connor
May 8, 2016·Biochimica Et Biophysica Acta·Christopher R Chitambar
Apr 23, 2002·Rapid Communications in Mass Spectrometry : RCM·Claire S AllardyceNeil Johnson
Jan 27, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Andrew J GhioJerrold L Abraham
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oyebode OlakanmiBradley E Britigan
Feb 25, 2020·Free Radical Biology & Medicine·Andrew J GhioMichael C Madden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.