Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

FEBS Letters
A M KomarovW B Weglicki

Abstract

The effect of exogenous Fe-citrate complex (Fe doses of 120 and 240 micromol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.

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Citations

Mar 31, 2004·Molecular Aspects of Medicine·Monica GalleanoSusana Puntarulo
Oct 2, 2001·Toxicology·M GalleanoS Puntarulo
Jul 20, 2001·Free Radical Biology & Medicine·E AldieriB Fubini
May 22, 2003·Free Radical Biology & Medicine·Akira I HidaShigeru Okada
May 8, 2004·Antioxidants & Redox Signaling·Tetsuhiko Yoshimura, Yashige Kotake
Oct 23, 2010·Journal of Cardiovascular Pharmacology·Atta U ShahbazKarl T Weber
Nov 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Atsushi SatoDavid D Gutterman
Feb 23, 2020·Scientific Reports·Gonçalo MesquitaMaria Salomé Gomes
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·W P WangC H Cho
Apr 11, 2002·Chemical Reviews·Tetsuo Nagano, Tetsuhiko Yoshimura
Apr 2, 2019·Journal of the American Chemical Society·Zhengying GuChengzhong Yu

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