Iron-chelator complexes as iron sources for early developing human erythroid precursors

Translational Research : the Journal of Laboratory and Clinical Medicine
J M LeimbergA M Konijn

Abstract

Developing erythroid cells are dependent on transferrin (Tf) to acquire iron in amounts sufficient for hemoglobin production. Previously, we showed that although these cells cannot grow in culture in the absence of Tf, ferritin (Ft) can substitute Tf to some extent and support the development of hemoglobin-containing cells. In the current study, we investigated the ability of various iron sources to replace Tf in cultures of normal human erythroid precursors. The results showed that whereas Ft and hemin supported erythroid cell proliferation and hemoglobinization in Tf-free cultures to some extent, ferric amonium citrate and iron complexed with several chelators had little or no effect. Although salicylaldehyde-isonicotinoyl-hydrazone, which is a tridentate lipid-soluble chelator, complexed with iron increased both cytosolic and mitochondrial labile iron pools, it failed to support heme synthesis and did not decrease the surface Tf receptors, suggesting that its iron is not recognized by the cells. Moreover, this iron-chelator complex did not support erythroid precursor proliferation and hemoglobinization. Thus, although under normal conditions, Tf is the major route of iron uptake, Ft and hemin, but not iron-chelator complexes...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S H OrkinP Leder
Aug 1, 1992·American Journal of Hematology·Y AbeH Nawata
Nov 1, 1986·Journal of Cellular Physiology·J D LaskeyH M Schulman
Mar 1, 1987·Journal of Cellular Physiology·E FibachE A Rachmilewitz
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J van RenswoudeR D Klausner
Oct 8, 1982·Biochimica Et Biophysica Acta·P PonkaA Wilczynska
Oct 13, 1995·The Journal of Biological Chemistry·W BreuerZ I Cabantchik
May 15, 1997·Analytical Biochemistry·S EpsztejnI Cabantchik
Apr 16, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B Scheiber, H Goldenberg
Apr 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·S THUNELL
Oct 4, 2006·Annual Review of Physiology·Nancy C Andrews, Paul J Schmidt

❮ Previous
Next ❯

Citations

Apr 15, 2011·Anemia·Eugenia Prus, Eitan Fibach
Dec 21, 2010·International Journal of Hematology·Clara Camaschella, Alessia Pagani
Mar 17, 2010·Biochimica Et Biophysica Acta·Daphne Q D Pham, Joy J Winzerling
May 28, 2008·British Journal of Haematology·Eugenia Prus, Eitan Fibach
Oct 28, 2019·Cellular and Molecular Life Sciences : CMLS·Ayelén D NigraVerónica S Santander
May 27, 2020·Mediators of Inflammation·Yikun LiDan Wan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Biological Chemistry
C FerreiraCarole Beaumont
The International Journal of Biochemistry & Cell Biology
Sonia Levi, Paolo Arosio
American Journal of Physiology. Cell Physiology
J FisherJ R Connor
© 2021 Meta ULC. All rights reserved