Nov 5, 2018

Iron deficiency affects early stages of embryonic hematopoiesis but not the endothelial to hematopoietic transition

BioRxiv : the Preprint Server for Biology
Maya ShvartsmanChristophe Lancrin

Abstract

Iron is an essential micronutrient for hematopoiesis and previous research suggested that iron deficiency in the pregnant female could cause anemia in the offspring. Since the development of all embryonic and adult blood cells begins in the embryo, we aimed to resolve the role of iron in embryonic hematopoiesis. For this purpose, we used an experimental system of mouse embryonic stem cells differentiation into embryonic hematopoietic progenitors. We modulated the iron status in cultures by adding either an iron chelator DFO for iron deficiency, or ferric ammonium citrate for iron excess, and followed the emergence of developing hematopoietic progenitors by flow cytometry. We found interestingly that iron deficiency by DFO did not block the endothelial to hematopoietic transition, the first step of hematopoiesis. However, it had a differential effect on the proliferation, survival and clonogenic capacity of hematopoietic progenitors. Surprisingly, iron deficiency affected erythro-myeloid Kitpos CD41+ progenitors significantly more than the primitive erythroid Kitneg CD41+. The Kitpos progenitors paradoxically died more, proliferated less and had more reduction in colony formation than Kitneg after 24 hours of DFO treatment. Kitp...Continue Reading

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Mentioned in this Paper

Embryo
Itga2b
Flow Cytometry
Research
Iron Chelating Agents
Iron Metabolism Disorders
Transferrin
Deferoxamine
Iron Deficiency Anemia
Iron Deficiency

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