PMID: 11925455Apr 2, 2002Paper

Iron deficiency decreases mitochondrial aconitase abundance and citrate concentration without affecting tricarboxylic acid cycle capacity in rat liver

The Journal of Nutrition
Kerry L Ross, R S Eisenstein

Abstract

Mitochondrial aconitase (m-acon) is the tricarboxylic acid (TCA) cycle enzyme that converts citrate to isocitrate. m-Acon mRNA is a potential target for regulation by iron regulatory proteins (IRPs), suggesting a link between dietary iron intake, m-acon synthesis, and energy metabolism. Our previous studies indicate that m-acon is one of a limited number of proteins that is down-regulated in iron-deficient liver. Here we use isolated hepatocytes to study the relationships among decreased m-acon abundance, TCA cycle function and cellular citrate concentration in iron deficiency. Rats were fed an iron-deficient (ID) (2 mg Fe/kg diet) diet, or they were pair-fed (PF) or freely fed (C) a control diet (50 mg Fe/kg diet) for up to 21 d. Hepatocyte total IRP activity was greater by d 2 in the ID group than in the C and PF groups and by d 10, the difference was maximal. Liver IRP activity was inversely correlated with m-acon abundance (r = -0.93, P < 0.0001). However, the decrease in m-acon abundance did not affect the ability of hepatocytes to oxidize 2-[(14)C]pyruvate or 1-[(14)C]acetate, indicating that TCA cycle capacity was not affected. Interestingly, by d 21, total liver citrate concentration was 40% lower in ID than in PF rats,...Continue Reading

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Citations

May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chai-Wan KimJay D Horton
Sep 5, 2018·Journal of Cachexia, Sarcopenia and Muscle·Magdalena DziegalaEwa Jankowska
Jun 24, 2008·The Journal of Nutrition·James P McClungAndrew J Young
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wing-Hang Tong, Tracey A Rouault
Mar 3, 2021·Archives of Toxicology·Amin GasmiGeir Bjørklund
May 6, 2003·The Journal of Nutrition·Richard S Eisenstein, Kerry L Ross
Jul 29, 2006·Biochimica Et Biophysica Acta·Michelle L WallanderRichard S Eisenstein

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