Iron-induced interleukin-6 gene expression: possible mediation through the extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways

Toxicology
Jisen DaiXi Huang

Abstract

Increased iron store in the body may increase the risk of many diseases such as cancer and inflammation. However, the precise pathogenic mechanism of iron has not yet been elucidated. In the present study, the early biological responses of cells to iron treatment were investigated in AP-1 luciferase reporter stably transfected mouse epidermal JB6 cells and primary rat hepatocytes. It was shown that water-soluble iron compounds, such as FeSO4 and Fe2(SO4)3, were more active in inducing AP-1 in JB6 cells than water-insoluble iron compounds, such as Fe2O3 and FeS. Iron stimulated mitogen-activated protein kinase (MAPK) family members of extracellular signal-regulated kinases (ERKs) and p38 MAPK but not c-jun NH2 terminal kinases (JNKs), both in JB6 cells and in primary rat hepatocytes, as determined by the phosphorylation assay. Interestingly, the increase in AP-1 luciferase activity by iron was inhibited by the pretreatment of the cells with PD98059, a specific MEK1 inhibitor, and SB202190, a p38 kinase inhibitor. Levels of interleukin-6 (IL-6), a pro-inflammatory cytokine, were increased in JB6 cells by iron in a dose-dependent manner. The increase in IL-6 and its mRNA by iron was also eliminated by the pretreatment of the cells...Continue Reading

References

Jan 1, 1992·Critical Reviews in Toxicology·T P Ryan, S D Aust
Jan 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M L BassettL W Powell
Nov 14, 1985·The New England Journal of Medicine·C NiederauG Strohmeyer
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z DongN H Colburn
Jan 1, 1996·Free Radical Biology & Medicine·S Toyokuni
Jan 16, 1998·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·J B Porter
May 12, 1998·Journal of Hepatology·A Pietrangelo
Oct 16, 1999·The Journal of Biological Chemistry·M DingV Vallyathan
Jan 25, 2000·The Journal of Biological Chemistry·S L Friedman
Apr 25, 2000·Annual Review of Medicine·S Sheth, G M Brittenham
Apr 9, 2001·The Journal of Biological Chemistry·C HuangX Shi
Jan 5, 2002·Free Radical Biology & Medicine·Xi HuangKrystyna Frenkel
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Antonello Pietrangelo
Mar 23, 2002·Free Radical Biology & Medicine·Mario ComportiLucia Ciccoli

❮ Previous
Next ❯

Citations

Dec 12, 2012·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Dale ViezelieneLeonid Ivanov
Jan 27, 2009·Immunobiology·Marija Poljak-BlaziDorotea Muck-Seler
Apr 19, 2008·Toxicology and Applied Pharmacology·Jianling WangM Firoze Khan
Aug 4, 2009·American Journal of Hematology·Rabaa M Al-RousanEric R Blough
Feb 16, 2011·Journal of Biochemical and Molecular Toxicology·Jane Kasten-JollyDavid A Lawrence
Feb 11, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Qing YangXi Huang
Jan 31, 2006·Free Radical Biology & Medicine·Jing WuXi Huang
Sep 20, 2005·Toxicology and Applied Pharmacology·M Firoze KhanJianling Wang
Jun 12, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Alexandr A NikonorovAnatoly V Skalny
Feb 26, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sudip BhattacharyyaParames C Sil
Apr 18, 2019·Reviews in Medical Virology·Zabihollah ShojaSomayeh Jalilvand
Mar 21, 2017·Oncogene·D BasuliS V Torti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.