Iron is not everything: unexpected complex metabolic responses between iron-cycling microorganisms.

The ISME Journal
Rebecca E CooperKirsten Küsel

Abstract

Coexistence of microaerophilic Fe(II)-oxidizers and anaerobic Fe(III)-reducers in environments with fluctuating redox conditions is a prime example of mutualism, in which both partners benefit from the sustained Fe-pool. Consequently, the Fe-cycling machineries (i.e., metal-reducing or -oxidizing pathways) should be most affected during co-cultivation. However, contrasting growth requirements impeded systematic elucidation of their interactions. To disentangle underlying interaction mechanisms, we established a suboxic co-culture system of Sideroxydans sp. CL21 and Shewanella oneidensis. We showed that addition of the partner's cell-free supernatant enhanced both growth and Fe(II)-oxidizing or Fe(III)-reducing activity of each partner. Metabolites of the exometabolome of Sideroxydans sp. CL21 are generally upregulated if stimulated with the partner´s spent medium, while S. oneidensis exhibits a mixed metabolic response in accordance with a balanced response to the partner. Surprisingly, RNA-seq analysis revealed genes involved in Fe-cycling were not differentially expressed during co-cultivation. Instead, the most differentially upregulated genes included those encoding for biopolymer production, lipoprotein transport, putresci...Continue Reading

References

Mar 1, 1979·Journal of Bacteriology·B Friedrich, B Magasanik
Mar 7, 1991·Journal of Theoretical Biology·J J Bull, W R Rice
Jan 1, 1984·Annual Review of Biochemistry·C W Tabor, H Tabor
May 13, 1999·International Journal of Systematic Bacteriology·K VenkateswaranK H Nealson
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
May 25, 2002·Environmental Science & Technology·Richard A RoyerBrian A Dempsey
Oct 9, 2002·Nature Biotechnology·John F HeidelbergClaire M Fraser
May 8, 2003·Applied Microbiology and Biotechnology·A K Lee, D K Newman
Nov 3, 2004·Advances in Microbial Physiology·Derek R LovleyKelly P Nevin
Dec 14, 2004·The Journal of Biological Chemistry·Shin KuriharaHideyuki Suzuki
Jul 28, 2005·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·J M StreitzA C Aufderheide
Mar 21, 2006·Journal of Bacteriology·Chandra N PatelMarcos A Oliveira
Jun 20, 2006·Journal of Evolutionary Biology·K R Foster, T Wenseleers
Sep 19, 2006·Nature Reviews. Microbiology·Karrie A WeberJohn D Coates
Dec 26, 2006·Applied and Environmental Microbiology·Galit Meshulam-SimonAlfred M Spormann
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Enrico MarsiliDaniel R Bond
Apr 2, 2008·Applied and Environmental Microbiology·Alexandra HambergerHarold L Drake
May 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Annelie PernthalerVictoria J Orphan
Jun 19, 2008·FEMS Microbiology Ecology·Marco ReicheKirsten Küsel
Aug 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Torsten RedaJudy Hirst
Feb 19, 2009·Environmental Microbiology·Pia K WüstHarold L Drake
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ece Karatan, Paula Watnick
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Nov 17, 2009·Applied and Environmental Microbiology·Julian BoschRainer U Meckenstock
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Robert S HartshorneDavid J Richardson
Jun 23, 2010·Annual Review of Microbiology·David EmersonJoyce M McBeth
Dec 7, 2010·Applied and Environmental Microbiology·Joyce M McBethDavid Emerson
Jul 5, 2011·Applied and Environmental Microbiology·Liang ShiJames K Fredrickson
Feb 23, 2012·Scientific Reports·Barbara Schoepp-CothenetWolfgang Nitschke
Mar 13, 2012·FEMS Microbiology Ecology·Anke HädrichKirsten Küsel
Mar 28, 2012·Nature Reviews. Microbiology·Karsten Zengler, Bernhard O Palsson
Apr 26, 2012·MBio·J L Sachs, A C Hollowell
Apr 27, 2012·Analytical Chemistry·Ralf TautenhahnGary Siuzdak
May 18, 2012·The ISME Journal·David R JohnsonMartin Ackermann
Jul 12, 2012·Frontiers in Microbiology·Eric E RodenJuergen Schieber
Aug 1, 2012·ACS Chemical Biology·Perry A Frey, George H Reed
Sep 22, 2012·Science·Anne W ThompsonJonathan P Zehr
Oct 10, 2012·Biochimica Et Biophysica Acta·Marianne Ilbert, Violaine Bonnefoy
Oct 17, 2012·Bioinformatics·Evguenia KopylovaHélène Touzet

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Methods Mentioned

BETA
RNA-seq
PCR
metabolomics profiling
metabolomic profiling

Software Mentioned

sortmerna
R framework for statistical analysis
bbmap
bbduk
edgeR
FastQC
subread
featureCounts
Xcalibur
samtools

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