Iron-related transcriptomic variations in CaCo-2 cells, an in vitro model of intestinal absorptive cells

Physiological Genomics
Celine ChicaultJean Mosser

Abstract

Regulation of iron absorption by duodenal enterocytes is essential for the maintenance of homeostasis by preventing iron deficiency or overload. Despite the identification of a number of genes implicated in iron absorption and its regulation, it is likely that further factors remain to be identified. For that purpose, we used a global transcriptomic approach, using the CaCo-2 cell line as an in vitro model of intestinal absorptive cells. Pangenomic screening for variations in gene expression correlating with intracellular iron content allowed us to identify 171 genes. One hundred nine of these genes are clustered into five types of expression profile. This is the first time that most of these genes have been associated with iron metabolism. Functional annotation of these five clusters indicates potential links between the immune response, proteolysis processes, and iron depletion. In contrast, iron overload is associated with cellular metabolism, especially that of lipids and glutathione involving redox function and electron transfer.

References

Nov 18, 1991·Biochimica Et Biophysica Acta·X Alvarez-HernandezJ Glass
May 27, 1982·Biochimica Et Biophysica Acta·N KojimaG W Bates
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J N FederR C Schatzman
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A WaheedW S Sly
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Sep 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·D M FrazerG J Anderson
Dec 1, 2001·Gastroenterology·S ParkkilaW S Sly
Dec 14, 2001·FEBS Letters·H GunshinM A Hediger
Apr 2, 2002·The Journal of Nutrition·Ligia A MartiniRichard J Wood
Sep 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nadia Hubert, Matthias W Hentze
Jan 28, 2003·Blood Cells, Molecules & Diseases·Kaashif A AhmadRobert E Fleming
Apr 24, 2003·Experimental Biology and Medicine·Aliye Uc, Bradley E Britigan
Mar 20, 2004·Experimental & Molecular Medicine·Ji-Won LeeKyu-Won Kim
Aug 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aliye UcBradley E Britigan
Aug 19, 2004·Journal of Cellular Physiology·Yukihisa TakahashiKazuo T Suzuki
Nov 20, 2004·Nature Reviews. Microbiology·Ulrich E Schaible, Stefan H E Kaufmann
Feb 3, 2005·Free Radical Biology & Medicine·Karen E Iles, Rui-Ming Liu
Mar 2, 2005·Best Practice & Research. Clinical Haematology·Antonella Roetto, Clara Camaschella
Apr 16, 2005·Molecular and Cellular Biology·Mark Steffen HippGunter Schmidtke
May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Birgit KindermannHannelore Daniel
Jun 2, 2005·Nitric Oxide : Biology and Chemistry·Pamela CornejoVirginia Fernández
Sep 7, 2005·Cell·Majid ShayeghiAndrew T McKie

❮ Previous
Next ❯

Citations

Aug 24, 2013·Journal of Cellular Biochemistry·Hélène BlanchardFrédérique Pédrono
Mar 1, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Perrine ZellerEric Leclerc
Jan 18, 2011·Biochimica Et Biophysica Acta·Anwar A Khan, John G Quigley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.