Iron sources used by the nonpathogenic lactic acid bacterium Lactobacillus sakei as revealed by electron energy loss spectroscopy and secondary-ion mass spectrometry.

Applied and Environmental Microbiology
Philippe DuhutrelMarie-Christine Champomier-Vergès

Abstract

Lactobacillus sakei is a lactic acid bacterium naturally found on meat. Although it is generally acknowledged that lactic acid bacteria are rare species in the microbial world which do not have iron requirements, the genome sequence of L. sakei 23K has revealed quite complete genetic equipment dedicated to transport and use of this metal. Here, we aimed to investigate which iron sources could be used by this species as well as their role in the bacterium's physiology. Therefore, we developed a microscopy approach based on electron energy loss spectroscopy (EELS) analysis and nano-scale secondary-ion mass spectrometry (SIMS) in order to analyze the iron content of L. sakei cells. This revealed that L. sakei can use iron sources found in its natural ecosystem, myoglobin, hemoglobin, hematin, and transferrin, to ensure long-term survival during stationary phase. This study reveals that analytical image methods (EELS and SIMS) are powerful complementary tools for investigation of metal utilization by bacteria.

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Citations

Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jean M MacklaimGregor Reid
Mar 4, 2011·Molecular Genetics and Genomics : MGG·O Ludvig NyquistIngolf F Nes
Jan 16, 2014·Journal of Food Science and Technology·Jayesh Jagannath AhireBhushan Liladhar Chaudhari
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Apr 12, 2015·Fish & Shellfish Immunology·Phillip DettleffVictor Martinez
Sep 8, 2017·Microorganisms·Monique Zagorec, Marie-Christine Champomier-Vergès
Jul 19, 2020·Applied and Environmental Microbiology·Emilie VerplaetseMarie-Christine Champomier-Vergès
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Ruan FourieCarolina H Pohl
Dec 1, 2015·Proteomes·Peter-Leon Hagedoorn
Sep 14, 2018·Frontiers in Microbiology·Chiara MontanariGiulia Tabanelli
Feb 26, 2020·Frontiers in Energy Research·Ian J CampbellJonathan J Silberg
Jan 16, 2020·Scientific Reports·Antonia María RomeroMaría Teresa Martínez-Pastor
Feb 6, 2021·Gut Microbes·Yohannes SeyoumChristèle Humblot

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