PMID: 9177174Jun 10, 1997Paper

Iron-sulfur cluster disassembly in the FNR protein of Escherichia coli by O2: [4Fe-4S] to [2Fe-2S] conversion with loss of biological activity

Proceedings of the National Academy of Sciences of the United States of America
N KhoroshilovaP J Kiley

Abstract

The transcription factor FNR (fumarate nitrate reduction) requires the presence of an iron-sulfur (Fe-S) cluster for its function as a global transcription regulator in Escherichia coli when oxygen becomes scarce. To define the oxidation state and type of Fe-S cluster present in the active form of FNR, we have studied anaerobically purified FNR with Mössbauer spectroscopy. Our data showed that this form of FNR contained a [4Fe-4S]2+ cluster (delta = 0.45 mm/s; DeltaEQ = 1.22 mm/s) and that the [4Fe-4S]2+ cluster was rapidly destroyed on exposure of FNR to air. Under these conditions, the yellow-green active form of FNR turned deep red; analysis of sulfide indicated that 70% of the labile sulfide was still present, suggesting that the Fe-S cluster had been converted into a different form. Little [3Fe-4S] cluster was, however, detected by EPR. According to Mössbauer spectroscopy, the [4Fe-4S]2+ cluster was converted in about 60% yield to a [2Fe-2S]2+ cluster (delta = 0.28 mm/s; DeltaEQ = 0.58 mm/s) following 17 min of exposure to air. The [2Fe-2S]2+ cluster form of FNR was much more stable to oxygen, but was unable to sustain biological activity (e.g., DNA binding). However, DNA binding and the absorption spectrum characteristic ...Continue Reading

References

Jul 8, 1975·Biochemical and Biophysical Research Communications·R MaskiewiczR G Bartsch
Apr 15, 1983·Archives of Biochemistry and Biophysics·H Beinert, A J Thomson
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N KhoroshilovaP J Kiley
Jan 1, 1994·Antonie van Leeuwenhoek·S Spiro

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Citations

Jul 5, 2008·Current Microbiology·Ismail Akyol, Claire A Shearman
Dec 23, 1998·FEBS Letters·B T BuiA Marquet
Feb 25, 2000·Mutation Research·S R RajskiJ K Barton
Jul 9, 1999·Biochimica Et Biophysica Acta·L Philippot, O Højberg
Apr 26, 2003·Current Opinion in Chemical Biology·Joseph T Jarrett
Jul 17, 1998·Current Opinion in Chemical Biology·M K Johnson
Jul 17, 1998·Current Opinion in Chemical Biology·J S ValentineE B Gralla
May 5, 1999·Current Opinion in Chemical Biology·H Beinert, P J Kiley
May 7, 2003·Current Opinion in Microbiology·Patricia J Kiley, Helmut Beinert
May 14, 1999·Current Opinion in Microbiology·G Sawers
May 16, 2012·Biochemistry·Angela S FleischhackerPatricia J Kiley
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Nov 20, 2004·Nature Reviews. Microbiology·Jeffrey Green, Mark S Paget
Sep 28, 2007·Natural Product Reports·Andrée MarquetMartin J Warren
May 3, 2000·Molecular Microbiology·M V ColomboT Ruiz-Argüeso
Mar 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Adrian J JervisJeffrey Green
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo ZhangMichael K Johnson
Nov 18, 2009·The Journal of Biological Chemistry·James EdwardsJames W B Moir
Jan 16, 2010·The Journal of Biological Chemistry·Anders BoysenMartin Overgaard
Nov 22, 2008·Antioxidants & Redox Signaling·F Wayne Outten, Elizabeth C Theil
Jan 5, 2011·Antioxidants & Redox Signaling·Regina Brigelius-Flohé, Leopold Flohé
Jan 14, 2012·Antioxidants & Redox Signaling·Jason C CrackNick E Le Brun
Jul 3, 2002·The EMBO Journal·Hugo Cruz-RamosRobert K Poole
Jan 13, 2000·Protein Science : a Publication of the Protein Society·S J LloydC D Stout
Feb 13, 2007·Journal of Bacteriology·Erin L Mettert, Patricia J Kiley
Nov 18, 2004·Journal of Bacteriology·Victoria R SuttonPatricia J Kiley
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·E V Morozkina, R A Zvyagilskaya
Jul 13, 2005·PLoS Biology·Kate S CarrollCarolyn R Bertozzi
Oct 15, 2013·Applied Biochemistry and Biotechnology·Yunlong YangFuqian Xu
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·N M BrownR S Eisenstein

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