PMID: 9428655Jan 15, 1998Paper

Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: average hydrophobicity and amino acid weight are involved in the adaptation of proteins to extreme environments

Biochimica Et Biophysica Acta
A Dello RussoV Bocchini

Abstract

The iron-superoxide dismutase in the thermoacidophilic archaeon Sulfolobus solfataricus has a homodimeric structure with a metal content of 0.7 atom of iron per subunit. The enzyme is insensitive to cyanide inhibition, sensitive to inactivation by H2O2 and is the most heat resistant SOD known so far being its half-life 2 h at 100 degrees C. Its primary structure was determined by a profitable combination of advanced mass spectrometry and automated sequence analysis of peptides obtained after cleavage of the purified protein. The enzyme subunit is composed of 210 amino acid residues accounting for a relative molecular mass of 24,112. It does not contain cysteine residues and has a high average of both hydrophobicity and amino acid weight. Vice versa, the hydrophobicity is lower in halophilic SODs. Therefore, it seems that the average hydrophobicity is involved in the adaptation of proteins to extreme environments. The multiple alignment of the primary structure of archaeal and thermophilic eubacterial SODs indicated that archaeal SODs evolved separately from the thermophilic eubacterial SODs and that halophiles originated from a gene different from that of thermophilic archaea.

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Citations

Jan 31, 2007·Applied Microbiology and Biotechnology·Yong-Zhi HeZhi-Yang Dong
Apr 18, 2008·Extremophiles : Life Under Extreme Conditions·Pasquale GrimaldiMariorosario Masullo
Oct 16, 2007·Applied and Environmental Microbiology·Moh'd A Salameh, Juergen Wiegel
Nov 19, 2013·World Journal of Microbiology & Biotechnology·Yanbing ZhuHuinong Cai
Apr 2, 2014·Chemical Reviews·Yuewei ShengJoan Selverstone Valentine
Dec 17, 2014·Journal of Theoretical Biology·Yanrui DingZhaolin Mou
Mar 11, 2008·Biochimica Et Biophysica Acta·Immacolata CastellanoEmmanuele De Vendittis
Aug 7, 2020·Antioxidants·Emilia PedoneDanila Limauro
Nov 14, 1998·Biochemical and Biophysical Research Communications·M A McLeanS G Sligar

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