PMID: 8600987Mar 15, 1996Paper

Iron transport across Caco-2 cell monolayers. Effect of transferrin, lactoferrin and nitric oxide

Biochimica Et Biophysica Acta
L SánchezJ H Brock

Abstract

Differentiated Caco-2 colon carcinoma cell monolayers grown in bicameral chambers have been used as an in vitro model to study the effect of different carrier molecules on mucosal iron transport. Transfer of iron across the monolayers in the apical-to-basolateral direction was greater from ferric lactoferrin than from iron citrate, while very little transport occurred from Fe-transferrin. However, a greater proportion of iron was retained by the cells when Fe-citrate was the donor. Caco-2 cells expressed transferrin receptors (n = 1.3 x 10(5) /cell; Ka = 2 x 10(8) l/mol), but binding of lactoferrin, though substantial in quantity, had an affinity too low to measure. When monolayers were incubated with 125I-labelled lactoferrin or transferrin some 125I-activity was transported, but almost all was TCA-soluble, suggesting that degradation products rather than intact protein were being transported. Addition of 10 microM S-nitroso-N-acetyl-D,L-penicillamine (SNAP), which produces nitric oxide (NO) in solution, caused a significant increase in iron transport from ferric citrate, but not from Fe-lactoferrin or Fe-transferrin. It is concluded that in this in vitro system lactoferrin but not transferrin enhances mucosal iron transport, ...Continue Reading

References

Dec 1, 1987·Pediatric Research·S J Fairweather-TaitM J Minski
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R D KlausnerK R Bridges

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Citations

Sep 24, 2008·Medical Microbiology and Immunology·Songtao LiNing Liu
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Agostina PietrantoniFabiana Superti
Jan 24, 1998·Critical Reviews in Clinical Laboratory Sciences·H A Goldenberg
Jan 17, 2017·Journal of Agricultural and Food Chemistry·Daniel RipollésLourdes Sánchez
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D BarisaniD Conte
Feb 26, 2003·Journal of Medical Virology·Assunta Maria Di BiaseFabiana Superti
Dec 8, 2009·Bioscience, Biotechnology, and Biochemistry·Celia ConesaLourdes Sánchez
Apr 12, 2000·Journal of the American College of Nutrition·C BosD Tomé

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