Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells

Nutrients
Xiaonan YuJie Feng

Abstract

This experiment was conducted to investigate the transport characteristics of iron from ferrous bisglycinate (Fe-Gly) in intestinal cells. The divalent metal transporter 1 (DMT1)-knockout Caco-2 cell line was developed by Crispr-Cas9, and then the cells were treated with ferrous sulfate (FeSO₄) or Fe-Gly to observe the labile iron pool and determine their iron transport. The results showed that the intracellular labile iron increased significantly with Fe-Gly or FeSO₄ treatment, and this phenomenon was evident over a wide range of time and iron concentrations in the wild-type cells, whereas in the knockout cells it increased only after processing with high concentrations of iron for a long time (p < 0.05). DMT1-knockout suppressed the synthesis of ferritin and inhibited the response of iron regulatory protein 1 (IRP-1) and IRP-2 to these two iron sources. The expression of peptide transporter 1 (PepT1) was not altered by knockout or iron treatment. Interestingly, the expression of zinc-regulated transporter (ZRT) and iron-regulated transporter (IRT)-like protein 14 (Zip14) was elevated significantly by knockout and iron treatment in wild-type cells (p < 0.05). These results indicated that iron from Fe-Gly was probably mainly tr...Continue Reading

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Citations

Jun 26, 2020·Metallomics : Integrated Biometal Science·Haoxuan DingJie Feng
Aug 28, 2020·Metallomics : Integrated Biometal Science·Haoxuan DingJie Feng
Feb 26, 2021·International Medical Case Reports Journal·Valentina TalaricoMarco Bertini
May 8, 2021·Metallomics : Integrated Biometal Science·Haoxuan DingJie Feng
Jun 3, 2021·Nutrients·Valentina TalaricoMarco Bertini

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Methods Mentioned

BETA
Fluorescence Imaging
immunoprecipitation assay
Protein Assay
electrophoresis
PCR
Fluorescence
fluorescence microscopy

Software Mentioned

SPSS

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