PMID: 9630620Jun 19, 1998Paper

Iron transport in K562 cells: a kinetic study using native gel electrophoresis and 59Fe autoradiography

Biochimica Et Biophysica Acta
Daniel Vyoral, Jiri Petrak

Abstract

The exact mechanisms of iron transport from endosomes to the target iron containing cellular proteins are currently unknown. To investigate this problem, we used the gradient gel electrophoresis and the sensitive detection of 59Fe by autoradiography to detect separate cellular iron compounds and their iron kinetics. Cells of human leukemic line K562 were labeled with [59Fe]transferrin for 30-600 s and cellular iron compounds in cell lysates were analyzed by native electrophoretic separation followed by 59Fe autoradiography. Starting with the first 30 s of iron uptake, iron was detectable in a large membrane bound protein complex (Band I) and in ferritin. Significant amounts of iron were also found in labile iron compound(s) with the molecular weight larger than 5000 as judged by ultrafiltration. Iron kinetics in these compartments was studied. Band I was the only compound with the kinetic properties of an intermediate. Transferrin, transferrin receptor and additional proteins of the approximate molecular weights of 130000, 66000 and 49000 were found to be present in Band I. The labile iron compounds and ferritin behaved kinetically as end products. No evidence for low molecular weight transport intermediates was found. These re...Continue Reading

References

Jul 26, 1990·Nature·G Warren
May 1, 1974·Analytical Biochemistry·R K Scopes
Oct 1, 1982·British Journal of Haematology·M J PippardC A Finch
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N LehmingM Ptashne
Mar 1, 1995·Blood Reviews·G J Kontoghiorghes, E D Weinberg
Mar 17, 1995·The Journal of Biological Chemistry·K D Kumble, A Kornberg
Mar 18, 1996·FEBS Letters·W BreuerZ I Cabantchik
Jul 31, 1996·Biochimica Et Biophysica Acta·P M Harrison, P Arosio
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W Hentze, L C Kühn
Jan 1, 1996·Free Radical Biology & Medicine·S Toyokuni
May 1, 1997·Clinical Rheumatology·J BelzuneguiM Figueroa
Aug 1, 1997·Nature Genetics·C Vulpe, J Gitschier
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S ParkkilaW S Sly
Apr 9, 1998·Biological Trace Element Research·D VyoralA Hradilek
Dec 22, 2012·The Journal of Biological Chemistry·Hee Yun SukChi-Wing Chow

❮ Previous
Next ❯

Citations

Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alex D SheftelRoland Lill
Mar 24, 2009·Transfusion Medicine Reviews·Mark R BleackleyRoss T A Macgillivray
Jan 22, 2008·Biochimica Et Biophysica Acta·Des R Richardson, Hiu Chuen Lok
Nov 29, 2005·Biochemical Pharmacology·C MouralianP Ponka
Apr 23, 2010·The Biochemical Journal·Maya ShvartsmanZ Ioav Cabantchik
Jul 25, 2014·The Biochemical Journal·Darius J R Lane, Des R Richardson
Jun 6, 2000·Biochimica Et Biophysica Acta·M GoralskaM C McGahan
Nov 2, 2019·Antioxidants & Redox Signaling·Dong-Hun BaeDes R Richardson
Sep 16, 2009·Molecular and Cellular Biology·Alex D SheftelRoland Lill
Jan 26, 2021·Free Radical Biology & Medicine·Vladimir CamarenaGaofeng Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.