PMID: 9555881Apr 29, 1998Paper

Iron uptake in Ustilago maydis: tracking the iron path

Journal of Bacteriology
O ArdonY Hadar

Abstract

In this study, we monitored and compared the uptake of iron in the fungus Ustilago maydis by using biomimetic siderophore analogs of ferrichrome, the fungal native siderophore, and ferrioxamine B (FOB), a xenosiderophore. Ferrichrome-iron was taken up at a higher rate than FOB-iron. Unlike ferrichrome-mediated uptake, FOB-mediated iron transport involved an extracellular reduction mechanism. By using fluorescently labeled siderophore analogs, we monitored the time course, as well as the localization, of iron uptake processes within the fungal cells. A fluorescently labeled ferrichrome analog, B9-lissamine rhodamine B, which does not exhibit fluorescence quenching upon iron binding, was used to monitor the entry of the compounds into the fungal cells. The fluorescence was found intracellularly 4 h after the application and later was found concentrated in two to three vesicles within each cell. The fluorescence of the fluorescently labeled FOB analog CAT18, which is quenched by iron, was visualized around the cell membrane after 4 h of incubation with the ferrated (nonfluorescent) compounds. This fluorescence intensity increased with time, demonstrating fungal iron uptake from the siderophores, which remained extracellular. We he...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A DancisR D Klausner
Dec 1, 1987·Journal of Bacteriology·B F MatzankeG Winkelmann
Jan 1, 1981·Annual Review of Biochemistry·J B Neilands
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B MeiS A Leong
Jan 1, 1996·Physiological Reviews·D M de SilvaJ Kaplan

❮ Previous
Next ❯

Citations

Feb 7, 2001·Microbiology·E LesuisseJ M Camadro
Jan 20, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Günther Winkelmann
Apr 8, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Brian Holinsworth, Jessica D Martin
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Katy Díaz PeraltaEugenio Sanfuentes
Aug 14, 2012·Chemical Society Reviews·Suban K SahooJohn F Callan
Feb 1, 2017·Clinical and Translational Imaging : Reviews in Nuclear Medicine and Molecular Imaging·Milos PetrikClemens Decristoforo
May 30, 2002·Biological Chemistry·Frank PetratUrsula Rauen
Feb 27, 2003·Molecular Microbiology·Daniel J Kosman
Aug 3, 2012·Metallomics : Integrated Biometal Science·Tengfei Zheng, Elizabeth M Nolan
Aug 29, 2013·Mycopathologia·Gérald LarcherJean-Philippe Bouchara
Feb 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Markus SchrettlHubertus Haas
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Aug 6, 2008·Annual Review of Phytopathology·Hubertus HaasB Gillian Turgeon
Mar 1, 2014·Microbial Biotechnology·E Ahmed, S J M Holmström
Sep 25, 2020·Scientific Reports·Joachim PfisterClemens Decristoforo
Oct 16, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Evgenia OlshvangAbraham Shanzer
Dec 1, 2020·Frontiers in Bioengineering and Biotechnology·Amira OrabySusanne Zibek
Jul 15, 2017·Chemistry : a European Journal·Jenny BesserglickAbraham Shanzer
Apr 4, 2021·Journal of Fungi·Janelle R RobinsonFelicia N Anike
Apr 24, 2002·Analytical Biochemistry·Breno P EspósitoZ Ioav Cabantchik
Jun 11, 2002·Archives of Biochemistry and Biophysics·Richard E Cowart
Jul 10, 1999·Clinical Microbiology Reviews·D H Howard
Jun 30, 2006·Biochimica Et Biophysica Acta·Caroline C Philpott
Feb 19, 2008·Mycological Research·Linda Johnson
Jan 11, 2007·Journal of the American Chemical Society·David MarguliesAbraham Shanzer
May 30, 1998·Journal of Medicinal Chemistry·R NudelmanA Shanzer
Mar 1, 2007·Inorganic Chemistry·Raghavendra KikkeriAbraham Shanzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.