Iron(II) and hydrogen peroxide detoxification by human H-chain ferritin. An EPR spin-trapping study

Biochemistry
Guanghua ZhaoN Dennis Chasteen

Abstract

Overexpression of human H-chain ferritin (HuHF) is known to impart a degree of protection to cells against oxidative stress and the associated damage to DNA and other cellular components. However, whether this protective activity resides in the protein's ability to inhibit Fenton chemistry as found for Dps proteins has never been established. Such inhibition does not occur with the related mitochondrial ferritin which displays much of the same iron chemistry as HuHF, including an Fe(II)/H(2)O(2) oxidation stoichiometry of approximately 2:1. In the present study, the ability of HuHF to attenuate hydroxyl radical production by the Fenton reaction (Fe(2+) + H(2)O(2) --> Fe(3+) + OH(-) + *OH) was examined by electron paramagnetic resonance (EPR) spin-trapping methods. The data demonstrate that the presence of wild-type HuHF during Fe(2+) oxidation by H(2)O(2) greatly decreases the amount of .OH radical produced from Fenton chemistry whereas the ferroxidase site mutant 222 (H62K + H65G) and human L-chain ferritin (HuLF) lack this activity. HuHF catalyzes the pairwise oxidation of Fe(2+) by the detoxification reaction [2Fe(2+) + H(2)O(2) + 2H(2)O --> 2Fe(O)OH(core) + 4H(+)] that occurs at the ferroxidase site of the protein, thereby ...Continue Reading

References

May 11, 1992·FEBS Letters·A TreffryP M Harrison
Dec 11, 1991·Biochimica Et Biophysica Acta·E R BaumingerA Treffry
Nov 13, 1990·European Journal of Biochemistry·B J Bolann, R J Ulvik
Aug 12, 1999·Journal of Structural Biology·N D Chasteen, P M Harrison
Aug 14, 1999·Progress in Biophysics and Molecular Biology·M Zámocký, F Koller
May 4, 2002·The Biochemical Journal·Fadi Bou-AbdallahN Dennis Chasteen
Jul 19, 2002·The Journal of Biological Chemistry·Fadi Bou-AbdallahN Dennis Chasteen
Jan 22, 2004·The Journal of Biological Chemistry·Nodar SurguladzeMichael G Fried
Jul 28, 2004·Journal of Molecular Biology·Fadi Bou-AbdallahN Dennis Chasteen
Mar 18, 2005·Journal of the American Chemical Society·Fadi Bou-AbdallahN Dennis Chasteen

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Citations

Oct 10, 2013·Inorganic Chemistry·Elizabeth C Theil
Aug 6, 2013·Journal of Agricultural and Food Chemistry·Tuo ZhangGuanghua Zhao
Jul 12, 2008·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jacob P BitounHuangen Ding
Jul 27, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elizabeth C TheilXiaofeng Liu
May 15, 2015·Biomolecules·Nikolaus Bresgen, Peter M Eckl
May 10, 2011·Parkinsonism & Related Disorders·Andrzej FriedmanJolanta Galazka-Friedman
Apr 13, 2010·Biochimica Et Biophysica Acta·Fadi Bou-Abdallah
Nov 25, 2011·Journal of Integrative Plant Biology·Andrea GalatroSusana Puntarulo
Jun 6, 2013·Chembiochem : a European Journal of Chemical Biology·Kourosh Honarmand EbrahimiWilfred R Hagen
Apr 11, 2014·Journal of Neurochemistry·Michelle Muller, Blair R Leavitt
Oct 14, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Abolfazl ZarjouJózsef Balla
Jul 11, 2006·Journal of Molecular Biology·Song-Gun KimYong-Hwan Lee
May 15, 2012·Free Radical Biology & Medicine·Xiayun LiaoGuanghua Zhao
Nov 5, 2014·Antioxidants & Redox Signaling·Giuseppina BarreraFabrizio Gentile
Feb 5, 2014·Food Chemistry·Rui YangGuanghua Zhao
Feb 18, 2014·The Journal of Physical Chemistry. a·Rozita LaghaeiRob D Coalson
Jan 1, 2007·Toxicology Mechanisms and Methods·Luis G Valerio
Nov 1, 2015·The Biochemical Journal·Paolo ArosioMaura Poli
Jul 25, 2006·The Journal of Biological Chemistry·Xiaofeng LiuElizabeth C Theil
Mar 2, 2017·Journal of Cancer Research and Clinical Oncology·Sen ChengFusheng Liu
Mar 21, 2018·Oncology Letters·Shihui HaoMin Shi
Apr 25, 2019·Heart Failure Reviews·Mihai LupuGabriela Adriana Filip
Feb 23, 2020·Scientific Reports·Gonçalo MesquitaMaria Salomé Gomes
Aug 18, 2020·Biochimica Et Biophysica Acta. General Subjects·Artem Melman, Fadi Bou-Abdallah
Jan 8, 2010·Inorganic Chemistry·Seung Jae Lee, Sarah L J Michel
Apr 17, 2021·Critical Reviews in Food Science and Nutrition·Han LiMing Du
Jun 22, 2017·Biochemistry·Matthew MehlenbacherFadi Bou-Abdallah
Oct 31, 2021·Journal of Food Science·Chenxi ZhangGuanghua Zhao

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