Irradiance and light dose influence histological localization of photodamage induced by photodynamic therapy with aminolaevulinic acid

The British Journal of Dermatology
R BissonnetteY Liu

Abstract

Photodynamic therapy (PDT) with aminolaevulinic acid (ALA) is used in many countries for the treatment of actinic keratoses. The objectives of the current study were to evaluate the influence of irradiance and light dose on the localization and extent of photodamage to mouse skin following ALA-PDT. In this study we evaluated the influence of irradiance and light dose on epidermal photodamage following ALA-PDT. Groups of hairless mice received an intraperitoneal injection of ALA followed 2 h later by exposure to 12 J cm(-2), 24 J cm(-2) or 48 J cm(-2) of unfiltered light from a slide projector. For each of these fluences, groups of mice were exposed at the following irradiances: 5 mW cm(-2), 20 mW cm(-2) or 40 mW cm(-2). Skin biopsies were performed 24 h and 72 h later. Histological localization of photodamage was influenced by irradiance and light dose. At high irradiance and low fluence, PDT photodamaged cells were mostly located in the upper area of the epidermis whereas at lower irradiance and high fluence the complete epidermis was necrotic and often absent 24 h after light exposure. Protoporphyrin IX (PpIX) fluorescence intensity was similar in the upper and lower region of the epidermis 2 h after ALA injection. The decrea...Continue Reading

References

Jul 1, 1997·Dermatologic Clinics·R Bissonnette, H Lui
Feb 25, 2000·Journal of the American Academy of Dermatology·K KalkaH Mukhtar

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Citations

Dec 1, 2005·Photodiagnosis and Photodynamic Therapy·Katie O'RiordanTayyaba Hasan
Jul 14, 2006·Journal of the European Academy of Dermatology and Venereology : JEADV·J BaptistaM Cochito
Jan 19, 2019·Journal of Dermatological Science·Thierry Passeron

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