Irradiated and CCl4 -Treated Bone Marrow-Derived Liver Macrophages Exhibit Different Gene Expression Patterns and Phenotypes

Scandinavian Journal of Immunology
Xu FanFuchu He

Abstract

Myeloid cells infiltrate into the liver and differentiate into macrophages in different liver-injury mouse models. However, the heterogeneity of bone marrow (BM) -derived LMs populations remains to be understood. To investigate this and understand the impact of the macrophage niche on the properties of recruited BM-derived macrophages, we used a non-myeloablation BM transplantation model to label and trace BM-derived LMs. Subsequently, we quantified the number of embryonic-derived liver-resident macrophages, BM-derived LMs, and total LMs, in CCl4 and irradiated acute liver injury mouse models respectively. Finally, we compared the cell fate, gene-expression patterns, chemokine signals, and surface markers of irradiated and CCl4 -treated BM-derived LMs. We observed that, as compared to CCl4, radiation generated a macrophage niche by depleting embryonic-derived liver-resident macrophages, and induced the recruitment of BM-derived LMs that further settled in the liver. Irradiated and CCl4 -treated BM-derived LMs are different with respect to their cell fates, gene-expression patterns, and chemokine expression and recruitment. They also have different surface markers shortly after differentiating from their progenitors. Our finding...Continue Reading

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