Irradiation-dependent effects on tumor perfusion and endogenous and exogenous hypoxia markers in an A549 xenograft model

International Journal of Radiation Oncology, Biology, Physics
Emmanouil FokasFrank Rose

Abstract

Hypoxia is a major determinant of tumor radiosensitivity, and microenvironmental changes in response to ionizing radiation (IR) are often heterogenous. We analyzed IR-dependent changes in hypoxia and perfusion in A549 human lung adenocarcinoma xenografts. Immunohistological analysis of two exogenously added chemical hypoxic markers, pimonidazole and CCI-103F, and of the endogenous marker Glut-1 was performed time dependently after IR. Tumor vessels and apoptosis were analyzed using CD31 and caspase-3 antibodies. Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and fluorescent beads (Hoechst 33342) were used to monitor vascular perfusion. CCI-103F signals measuring the fraction of hypoxic areas after IR were significantly decreased by approximately 50% when compared with pimonidazole signals, representing the fraction of hypoxic areas from the same tumors before IR. Interestingly, Glut-1 signals were significantly decreased at early time point (6.5 h) after IR returning to the initial levels at 30.5 h. Vascular density showed no difference between irradiated and control groups, whereas apoptosis was significantly induced at 10.5 h post-IR. DCE-MRI indicated increased perfusion 1 h post-IR. The discrepancy between t...Continue Reading

References

Jul 1, 1994·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·P L Olive
Dec 21, 2000·International Journal of Radiation Oncology, Biology, Physics·A S LjungkvistA J Van Der Kogel
Aug 17, 2002·International Journal of Radiation Oncology, Biology, Physics·Anna S E LjungkvistJuliana Denekamp
May 17, 2003·Science·Monica Garcia-BarrosRichard Kolesnick
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Oct 8, 2003·International Journal of Radiation Biology·C PetersenM Baumann
Jul 16, 2004·Seminars in Radiation Oncology·Peter Vaupel
Sep 1, 2004·International Journal of Radiation Oncology, Biology, Physics·Hanneke W M van LaarhovenAlbert J van Der Kogel
Dec 1, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hisataka KobayashiMartin W Brechbiel
May 26, 2005·International Journal of Radiation Oncology, Biology, Physics·Anna S E LjungkvistAlbert J van der Kogel
Aug 16, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Brita Singers SørensenMichael R Horsman
Oct 4, 2005·International Journal of Radiation Oncology, Biology, Physics·Nathalie CrokartBernard Gallez
Feb 24, 2006·Radiation Research·Anna S E LjungkvistAlbert J van der Kogel
May 26, 2006·Nature·Jacques PouysségurNathalie M Mazure
Jul 21, 2006·The British Journal of Radiology·A Y IsaJ J Homer
Sep 1, 2006·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Arnulf MayerPeter Vaupel
Apr 19, 2007·Cancer Metastasis Reviews·Benjamin J MoellerMark W Dewhirst
May 22, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Brita Singers SørensenMichael R Horsman
Jun 15, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Mark W DewhirstBenjamin Moeller
Jul 3, 2007·Cancer Metastasis Reviews·Debbie Liao, Randall S Johnson
Mar 21, 2008·International Journal of Cancer. Journal International Du Cancer·Morten BuskJens Overgaard
Jul 1, 2008·The Oncologist·Adrianus J de LangenMark Lubberink

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Citations

Aug 28, 2012·Cancer Immunology, Immunotherapy : CII·Katja HattarUlrich Grandel
May 11, 2012·Journal of the National Cancer Institute·Sergey V KozinRakesh K Jain
Oct 4, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Birgitte Mayland HavelundAnders Jakobsen
Jul 9, 2013·Biochemical and Biophysical Research Communications·Masaki NaganeOsamu Inanami
Dec 19, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Florentine S B SubtilJörg Hänze
Feb 13, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Keita SaitoMurali C Krishna
May 10, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Daisuke KokuryoTsuneo Saga
Mar 12, 2019·Biomedical Physics & Engineering Express·A G OrlovaI V Turchin

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