Abstract
It has been shown that plasminogen activator inhibitor type-1 (PAI-1) and vascular endothelial growth factor (VEGF) are involved in neo-angiogenesis. The aim of this study was to investigate the irradiation-induced regulation of PAI-1 and VEGF in squamous cell carcinomas of the head and neck (SCCHN) cell lines of varying radiation sensitivity. Six cell lines derived from SCCHN were investigated in vitro. The colorimetric AlamarBlue assay was used to detect metabolic activity of cell lines during irradiation as a surrogate marker for radiation sensitivity. PAI-1 and VEGF secretion levels were measured by enzyme-linked immunosorbent assay 24, 48, and 72 h after irradiation with 0, 2, 6, and 10 Gy. The direct radioprotective effect of exogenous PAI-1 was measured using the clonogenic assay. For regulation studies, transforming growth factor-beta1 (TGF-beta1), hypoxia-inducible factor-1alpha (HIF-1alpha), hypoxia-inducible factor-2alpha (HIF-2alpha), or both HIF-1alpha and HIF-2alpha were downregulated using siRNA. Although baseline levels varied greatly, irradiation led to a comparable dose-dependent increase in PAI-1 and VEGF secretion in all six cell lines. Addition of exogenous stable PAI-1 to the low PAI-1-expressing cell line...Continue Reading
References
Aug 1, 1990·The Journal of Cell Biology·M S PepperJ D Vassalli
Apr 11, 2000·International Journal of Radiation Biology·W ZhaoM E Robbins
May 16, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·B D SmithB G Haffty
Aug 5, 2000·International Journal of Radiation Oncology, Biology, Physics·D M AebersoldV Djonov
Mar 27, 2001·The Journal of Cell Biology·K BajouA Noël
Jun 26, 2001·Oncogene·J S ParkP Dent
Dec 19, 2001·British Journal of Cancer·C HessM Pruschy
Mar 16, 2002·The Cancer Journal·Vinay K GuptaRalph R Weichselbaum
Apr 18, 2003·Oncogene·Yasushi SuganoKyoichi Inoue
Feb 26, 2004·Experimental Cell Research·Geir Olav HjortlandOlav Engebraaten
May 12, 2004·FEBS Letters·Charles K BrownHelena J Mauceri
May 18, 2004·Cancer Cell·Benjamin J MoellerMark W Dewhirst
Aug 3, 2004·Oncogene·Khalid BajouAgnès Noel
Aug 16, 2005·Cancer Cell·Benjamin J MoellerMark W Dewhirst
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jurre HagemanRobert P Coppes
Sep 30, 2005·Journal of Neuro-oncology·K E HovingaW R van Furth
Dec 22, 2006·Cancer·Rahul R ParikhBruce G Haffty
May 23, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Ala YarominaMichael Baumann
Aug 1, 2007·BMC Cancer·Daniela SchillingMichael Molls
Aug 9, 2007·Oncogene·A WesolowskaB Kaminska
Dec 14, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Christine BayerMarkus Adam
Citations
Feb 22, 2012·PloS One·Daniela SchillingGabriele Multhoff
May 9, 2012·PloS One·Rym AbderrahmaniFabien Milliat
Jul 16, 2011·AJNR. American Journal of Neuroradiology·M T TruongO Sakai
Jan 5, 2011·European Journal of Radiology·Lin-Feng ZhengGui-Xiang Zhang
May 15, 2015·Head & Neck·Yueh-Chun LeeWen-Wei Chang
Apr 15, 2011·International Journal of Radiation Oncology, Biology, Physics·Ala YarominaDaniel Zips
Jun 13, 2012·International Journal of Radiation Oncology, Biology, Physics·Christine BayerGabriele Multhoff
Jul 8, 2016·Oncotarget·Miguel Angel PavónRamón Mangues