Irreversible metabolic transitions: the glucose 6-phosphate metabolism in yeast cell-free extracts

Biochemical and Biophysical Research Communications
M A Coevoet, J F Hervagault

Abstract

The steady-state and dynamic behavior of a partial glycolytic reaction sequence are investigated in cell-free extracts of yeast. Pyruvate kinase, adenylate kinase and glucose 6-phosphate isomerase cooperate to a multienzyme system centered around the 6-phosphofructokinase (6-PFK) and fructose 1,6-bisphosphatase (FBPase) cycle. The reaction system operates under thermodynamically open conditions maintained by a continuous supply of substrates, i.e., glucose 6-phosphate (Glc6P), ATP and phosphoenolpyruvate (PPrv) in a flow-through reaction chamber. Appropriate conditions lead to the occurrence of (two) coexisting and markedly different time-independent states in the metabolite concentrations and fluxes. For particular experimental conditions, changes in the influx adenylic energy charge, [AEC]IN, may cause transitions between these alternative steady states which are either reversible as it occurs in classical hysteresis phenomena, or, more importantly, irreversible (irreversible transitions, IT) where the system is not able to switch back to its previous state even when the perturbation is reverted. The emergence of these irreversible transitions do not result from artificial or non-realistic experimental constraints, but are a ...Continue Reading

References

Jan 1, 1978·Current Topics in Cellular Regulation·E R Stadtman, P B Chock
Jan 1, 1978·Genetics·D CliftonD G Fraenkel
Aug 21, 1979·Journal of Theoretical Biology·K Tornheim
Jan 1, 1976·Annual Review of Biophysics and Bioengineering·A Goldbeter, S R Caplan
May 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·M JusićH Holzer
Jan 1, 1991·European Journal of Biochemistry·W Schellenberger, J F Hervagault
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J F HervagaultJ Ross
Mar 30, 1990·European Journal of Biochemistry·K EschrichE Hofmann
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J E Lisman
Jan 1, 1985·Advances in Enzyme Regulation·E HofmannW Schellenberger
Dec 16, 1987·Biochemical and Biophysical Research Communications·A Cimino, J F Hervagault
Aug 21, 1987·Journal of Theoretical Biology·J F Hervagault, S Canu
Mar 17, 1986·European Journal of Biochemistry·J H HofmeyrK J van der Merwe
Oct 1, 1973·Journal of Theoretical Biology·H S HahnJ Ross
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·H EisenF Jacob
Jan 1, 1982·Methods in Enzymology·E Hofmann, G Kopperschläger
Aug 1, 1982·Quarterly Reviews of Biophysics·A Goldbeter, D E Koshland
Jan 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·B Hess
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A Goldbeter, D E Koshland
Mar 7, 1982·Journal of Theoretical Biology·J Ricard, J M Soulie
Jul 15, 1957·Proceedings of the National Academy of Sciences of the United States of America·A Novick, M Weiner
Aug 5, 1988·Biotechnology and Bioengineering·M OkamotoK Hayashi
Dec 1, 1970·The Journal of Membrane Biology·R BlumenthalR Lefever

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Citations

Oct 23, 2012·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Mark SteckerMatthew Stevenson
Jul 29, 1999·Biophysical Journal·H Berry, V Larreta-Garde
Nov 5, 1999·Trends in Biochemical Sciences·M Laurent, N Kellershohn
Aug 17, 2002·Journal of Theoretical Biology·Veronique Larreta-Garde, Hugues Berry
Oct 11, 2007·Journal of Agricultural and Food Chemistry·Wun-Yuan LinTzu-Ming Pan

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