PMID: 9555072May 23, 1998Paper

Irreversible phase transition of firefly luciferase: contrasting effects of volatile anesthetics and myristic acid

Biochimica Et Biophysica Acta
I Ueda, A Suzuki

Abstract

Firefly luciferase (FFL) has been used as a lipid-free protein model to study direct interaction of anesthetics with proteins. FFL emits a burst of light when luciferin and ATP are added in the presence of oxygen. Volatile anesthetics inhibited FFL at mM ranges, while myristic acid inhibited it at microM range. Despite the large difference, octanol/water partition coefficients of both myristic acid and halothane are 199. Differential scanning calorimetry (DSC) showed that thermal transition occurred at 38.5 degreesC with excess enthalpy of denaturation of 91.9 kcal mol-1. The transition, however, was irreversible. According to the irreversible transition kinetics, the anesthetic effects were evaluated by the temperature where the irreversible transition is half completed (T1/2). Volatile anesthetics decreased T1/2 at mM ranges, while myristic acid and oxyluciferyladenylate (luciferin competitor) increased it at microM ranges. Luciferin is a heterocyclic carboxylate and acylates AMP. Carboxyl group of myristic acid appears to make a high affinity contact to the luciferin-recognition sites. The induced-fit theory states that binding of substrates induces the enzyme into high-energy transition state. Myristic acid stabilized FFL a...Continue Reading

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Citations

May 18, 2000·Biochemical and Biophysical Research Communications·L K BrennanK Ohlendieck
May 16, 2000·Chemistry and Physics of Lipids·I Ueda, T Yoshida
Mar 20, 1999·Biochimica Et Biophysica Acta·J W TannerP A Liebman
Jun 27, 2000·Journal of Biotechnology·V Y GrinbergV B Tolstoguzov
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Satoshi Inouye
Sep 11, 2009·Colloids and Surfaces. B, Biointerfaces·Makoto NishimotoHitoshi Matsuki
Jan 18, 2005·Biochimica Et Biophysica Acta·Kô TakeharaIssaku Ueda
Mar 18, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shinya YamasakiKô Takehara
Oct 1, 2009·Accounts of Chemical Research·Satyavani VemparalaMichael L Klein

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