Is amniotic fluid of women with uncomplicated term pregnancies free of bacteria?

American Journal of Obstetrics and Gynecology
Eva Maria RehbinderKnut Rudi

Abstract

The "sterile womb" paradigm is debated. Recent evidence suggests that the offspring's first microbial encounter is before birth in term uncomplicated pregnancies. The establishment of a healthy microbiota early in life might be crucial for reducing the burden of diseases later in life. We aimed to investigate the presence of a microbiota in sterilely collected amniotic fluid in uncomplicated pregnancies at term in the Preventing Atopic Dermatitis and Allergies in children (PreventADALL) study cohort. Amniotic fluid was randomly sampled at cesarean deliveries in pregnant women in 1 out of 3 study sites included in the PreventADALL study. From 65 pregnancies at term, where amniotic fluid was successfully sampled, we selected 10 from elective (planned, without ongoing labor) cesarean deliveries with intact amniotic membranes and all 14 with prior rupture of membranes were included as positive controls. Amniotic fluid was analyzed by culture-independent and culture-dependent techniques. The median (min-max) concentration of prokaryotic DNA (16S rRNA gene copies/mL; digital droplet polymerase chain reaction) was low for the group with intact membranes [664 (544-748)]-corresponding to the negative controls [596 (461-679)], while the ...Continue Reading

Associated Clinical Trials

May 20, 2015·Karin C. Lødrup Carlsen

Citations

Jun 5, 2019·Nutrients·Agnieszka Gadecka, Anna Bielak-Zmijewska
Jul 31, 2019·American Journal of Physiology. Endocrinology and Metabolism·Tomás CerdóCristina Campoy
Oct 11, 2019·Journal of Epidemiology and Community Health·Gwinyai MasukumeAli S Khashan
Mar 3, 2020·Frontiers in Pediatrics·Samuel J Gentle, Charitharth Vivek Lal
Jul 28, 2020·International Journal of Molecular Sciences·Rosita GabbianelliJoan G Lalor
Feb 13, 2020·F1000Research·Eamonn M M Quigley, Prianka Gajula
Dec 24, 2018·Birth Defects Research·Kathryn A KnoopRodney D Newberry
Feb 28, 2020·MSphere·Kevin R TheisNardhy Gomez-Lopez
Jun 25, 2019·Frontiers in Microbiology·Lisa F StinsonJeffrey A Keelan
May 12, 2021·Nature Microbiology·Katherine M KennedyThorsten Braun
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Ada RotaMichela Corrò
Jul 7, 2021·Journal of Perinatal Medicine·Eunjung JungChaur-Dong Hsu
May 1, 2020·American Journal of Obstetrics and Gynecology·Yu LiuHuixia Yang

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