Is autopsy tissue a valid control for epilepsy surgery tissue in microRNA studies?

Epilepsia Open
Paolo RonconMichele Simonato

Abstract

MicroRNAs (miRNAs) are differentially expressed in the brain under pathologic conditions and may therefore represent both therapeutic targets and diagnostic or prognostic biomarkers for neurologic diseases, including epilepsy. In fact, miRNA expression profiles have been investigated in the hippocampi of patients with epilepsy in comparison with control, nonepileptic cases. Unfortunately, the interpretation of these data is difficult because surgically resected epileptic tissue is generally compared with control tissue obtained from autopsies. To challenge the validity of this approach, we performed an miRNA microarray on the laser microdissected granule cell layer of the human hippocampus obtained from surgical samples of patients with epilepsy, autoptic nonepileptic controls, and patients with autoptic epilepsy, using the latter as internal control. Unfortunately, it is extremely difficult to collect autopsy material from documented epilepsy individuals who died of non-epilepsy-related causes-we found only two such cases. However, hierarchical clustering of all samples showed that those obtained from autopsies of patients with epilepsy segregated with the other autoptic samples (controls) and not with the bioptic tissues from...Continue Reading

References

Jun 17, 2006·Annual Review of Neuroscience·Xinwei CaoFred H Gage
Feb 8, 2011·Journal of Biotechnology·Regina MargisCarlos R M Rieder
Sep 29, 2011·The American Journal of Pathology·Eva M Jimenez-MateosDavid C Henshall
Apr 27, 2012·Cellular and Molecular Life Sciences : CMLS·Anne A KanR Jeroen Pasterkamp
Jun 12, 2012·Nature Medicine·Eva M Jimenez-MateosDavid C Henshall
Oct 23, 2013·PloS One·Anna Maria BotKatarzyna Lukasiuk
Jun 27, 2014·Epilepsia·Elsa RossignolAlbert J Becker
Jun 16, 2016·Lancet Neurology·Asla PitkänenHeinz Beck

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Methods Mentioned

BETA
chip
Feature Extraction

Software Mentioned

Feature Extraction
GeneSpring GX

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