Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?

Animal Reproduction Science
Junwei LiInmaculada Parrilla

Abstract

This study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10 mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2 were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17 °C overnight before being frozen in 0.5 mL straws using a standard protocol. Sperm motility (total and progressive) deteriorated (P < 0.05) when P1- or P2-sperm when incubated overnight in SP from post-SRF, while sperm viability differed between P1 and P2 (P < 0.05) regardless of the SP they were incubated in. Post-thaw sperm quality and functionality differed between P1 and P2, regardless of the SP used for overnight pre-freezing incubation. Post-thaw motility (P < 0.05) and viability (P < 0.01), as well as plasma membrane fluidity (P < 0.05) or lipid peroxidation values (P < 0.01) were best in P1 sperm compared to those of P2. The protein pro...Continue Reading

Citations

Sep 28, 2018·Molecular & Cellular Proteomics : MCP·Cristina Pérez-PatiñoJordi Roca
Jan 27, 2019·Scientific Reports·Cristina Pérez-PatiñoInmaculada Parrilla
Feb 24, 2020·Theriogenology·Jordi RocaInmaculada Parrilla
Jan 1, 2019·Molecular & Cellular Proteomics : MCP·Cristina Pérez-PatiñoJordi Roca
Feb 1, 2020·Animal Reproduction Science·Lisa HöfnerDagmar Waberski
Feb 12, 2021·Reproduction in Domestic Animals = Zuchthygiene·Camila R C de BritoCarine D Corcini
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Estíbaliz LacalleFelipe Martínez-Pastor
Jul 3, 2021·Animals : an Open Access Journal From MDPI·José Néstor CaamañoCarlos Olegario Hidalgo

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