Is bursting more effective than spiking in evoking pituitary hormone secretion? A spatiotemporal simulation study of calcium and granule dynamics

American Journal of Physiology. Endocrinology and Metabolism
Alessia TagliaviniM G Pedersen

Abstract

Endocrine cells of the pituitary gland secrete a number of hormones, and the amount of hormone released by a cell is controlled in large part by the cell's electrical activity and subsequent Ca(2+) influx. Typical electrical behaviors of pituitary cells include continuous spiking and so-called pseudo-plateau bursting. It has been shown that the amplitude of Ca(2+) fluctuations is greater in bursting cells, leading to the hypothesis that bursting cells release more hormone than spiking cells. In this work, we apply computer simulations to test this hypothesis. We use experimental recordings of electrical activity as input to mathematical models of Ca(2+) channel activity, buffered Ca(2+) diffusion, and Ca(2+)-driven exocytosis. To compare the efficacy of spiking and bursting on the same cell, we pharmacologically block the large-conductance potassium (BK) current from a bursting cell or add a BK current to a spiking cell via dynamic clamp. We find that bursting is generally at least as effective as spiking at evoking hormone release and is often considerably more effective, even when normalizing to Ca(2+) influx. Our hybrid experimental/modeling approach confirms that adding a BK-type K(+) current, which is typically associated ...Continue Reading

References

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Citations

Nov 14, 2016·Mathematical Biosciences·Morten Gram PedersenFrancesco Montefusco
Jan 12, 2017·Biophysical Journal·Alessia Tagliavini, Morten Gram Pedersen
Jun 8, 2017·Biophysical Journal·Francesco MontefuscoMorten Gram Pedersen
Jun 28, 2017·Molecular and Cellular Endocrinology·Patrick A FletcherStanko S Stojilkovic
May 17, 2018·Stress : the International Journal on the Biology of Stress·Julia K GjerstadFrancesca Spiga
Apr 7, 2020·PLoS Computational Biology·David M RichardsJoel Tabak
Dec 6, 2019·International Journal of Molecular Sciences·Francesco Montefusco, Morten G Pedersen
Dec 3, 2021·The Journal of Physiology·Peter J DuncanMichael J Shipston

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