Is cadmium chloride-induced inter-sertoli tight junction permeability barrier disruption a suitable in vitro model to study the events of junction disassembly during spermatogenesis in the rat testis?

Endocrinology
N P Chung, C Y Cheng

Abstract

The events of germ cell movement during spermatogenesis are composed of intermittent phases of junction disassembly and reassembly. Although primary Sertoli cells cultured in vitro can be used to study junction reassembly, an in vitro model to study the events of junction disassembly is still lacking. We have assessed whether the CdCl(2)-induced inter-Sertoli tight junction (TJ) permeability barrier disruption in vitro can fill this gap. When Sertoli cells (1.2 x 10(6) cells/cm(2)) were cultured on Matrigel-coated bicameral units to allow the assembly of inter-Sertoli TJs, it was manifested by a steady rise in transepithelial electrical resistance across the Sertoli cell epithelia. Exposure of these cells on day 1 (i.e. 24 h after their isolation) to CdCl(2) at 5-10 microM for 8 h could perturb the inter-Sertoli TJ assembly dose dependently without any apparent cytotoxicity. Likewise, when cells were exposed to CdCl(2) (0.1-5 microM) on day 4 for 8 h after inter-Sertoli TJs were already assembled, CdCl(2) also perturbed the maintenance of inter-Sertoli TJ permeability barrier dose dependently without signs of cell cytotoxicity. Although the perturbed inter-Sertoli TJs were not capable of resealing even after the removal of CdCl...Continue Reading

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Citations

Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Weiliang XiaC Yan Cheng
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Elissa W P WongC Yan Cheng
Jan 15, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen H N YanC Yan Cheng
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Carla M K MorrowRex A Hess
May 17, 2008·Pharmacological Reviews·Dolores D MrukC Yan Cheng
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
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May 9, 2012·Toxicology and Applied Pharmacology·Cendrine Geoffroy-SiraudinMarie-Roberte Guichaoua
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Aug 18, 2009·Molecular and Cellular Endocrinology·C Yan ChengDolores D Mruk
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Jun 10, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qiaozhen YangGang Li
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Mar 7, 2014·Seminars in Cell & Developmental Biology·Kin Lam FokHsiao Chang Chan
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Apr 2, 2004·Biology of Reproduction·Nikki P Y Lee, C Yan Cheng
Feb 28, 2003·Biology of Reproduction·Wing-Yee LuiC Yan Cheng

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